Anti-YWHAG 항체 (CAB3043)

Antibody Name:	Anti-YWHAG Antibody
Antibody SKU:	CAB3043
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide of human YWHAG

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-251MG, K-562, HeLa, Mouse brain, Mouse heart

Immunogen:	A synthetic peptide of human YWHAG
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	7532
Uniprot:	P61981
Cellular Location:	Cytoplasm
Calculated MW:	28kDa
Observed MW:	28kDa

Synonyms:

YWHAG, 14-3-3GAMMA, PPP1R170

Background:

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways.

UniProt Protein Function:	14-3-3 gamma: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Homodimer. Interacts with SAMSN1. Interacts with RAF1, SSH1 and CRTC2/TORC2. Interacts with ABL1 (phosphorylated form); the interaction retains it in the cytoplasm. Interacts with GAB2. Interacts with MDM4 (phosphorylated); negatively regulates MDM4 activity toward TP53. Interacts with PKA-phosphorylated AANAT. Highly expressed in brain, skeletal muscle, and heart. Belongs to the 14-3-3 family.
UniProt Protein Details:	Protein type:Adaptor/scaffold Chromosomal Location of Human Ortholog: 7q11.23 Cellular Component: cytoplasmic vesicle membrane; cytosol; focal adhesion; membrane Molecular Function:insulin-like growth factor receptor binding; protein binding; protein kinase C binding Biological Process: G2/M transition of mitotic cell cycle; regulation of neuron differentiation; regulation of synaptic plasticity
NCBI Summary:	This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways. [provided by RefSeq, Jul 2008]
UniProt Code:	P61981
NCBI GenInfo Identifier:	48428721
NCBI Gene ID:	7532
NCBI Accession:	P61981.2
UniProt Secondary Accession:	P61981,O70457, P35214, Q6FH52, Q9UDP2, Q9UN99,
UniProt Related Accession:	P61981
Molecular Weight:	28,303 Da
NCBI Full Name:	14-3-3 protein gamma
NCBI Synonym Full Names:	tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein gamma
NCBI Official Symbol:	YWHAG
NCBI Official Synonym Symbols:	PPP1R170; 14-3-3GAMMA
NCBI Protein Information:	14-3-3 protein gamma
UniProt Protein Name:	14-3-3 protein gamma
UniProt Synonym Protein Names:	Protein kinase C inhibitor protein 1; KCIP-1
Protein Family:	14-3-3 protein
UniProt Gene Name:	YWHAG
UniProt Entry Name:	1433G_HUMAN

	Western blot analysis of extracts of various cell lines, using YWHAG antibody (CAB3043) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded Human colon using YWHAG Rabbit pAb (CAB3043) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded Rat spleen using YWHAG Rabbit pAb (CAB3043) at dilution of 1:100 (40x lens).

Antibodies

Anti-YWHAG Antibody (CAB18060)[KO Validated]

Anti-14-3-3 gamma Antibody (CAB9162)

YWHAG Antibody (PACO05315)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)