Anti-YKL-40 / CHI3L1 Antibody (CAB3166)
- SKU:
- CAB3166
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Death
Frequently bought together:
Description
| Antibody Name: | Anti-YKL-40 / CHI3L1 Antibody |
| Antibody SKU: | CAB3166 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 22-240 of human YKL-40 / CHI3L1 (NP_001267.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | MCF7, HT-29 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 22-240 of human YKL-40 / CHI3L1 (NP_001267.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | YKLV CYYT SWSQ YREG DGSC FPDA LDRF LCTH IIYS FANI SNDH IDTW EWND VTLY GMLN TLKN RNPN LKTL LSVG GWNF GSQR FSKI ASNT QSRR TFIK SVPP FLRT HGFD GLDL AWLY PGRR DKQH FTTL IKEM KAEF IKEA QPGK KQLL LSAA LSAG KVTI DSSY DIAK ISQH LDFI SIMT YDFH GAWR GTTG HHSP LFRG QEDA SPDR FSNT DYA |
| Gene ID: | 1116 |
| Uniprot: | P36222 |
| Cellular Location: | Cytoplasm, Endoplasmic reticulum, Secreted, extracellular space, perinuclear region |
| Calculated MW: | 42kDa |
| Observed MW: | 43kDa |
| Synonyms: | CHI3L1, ASRT7, CGP-39, GP-39, GP39, HC-gp39, HCGP-3P, YKL-40, YKL40, YYL-40, hCGP-39 |
| Background: | Chitinases catalyze the hydrolysis of chitin, which is an abundant glycopolymer found in insect exoskeletons and fungal cell walls. The glycoside hydrolase 18 family of chitinases includes eight human family members. This gene encodes a glycoprotein member of the glycosyl hydrolase 18 family. The protein lacks chitinase activity and is secreted by activated macrophages, chondrocytes, neutrophils and synovial cells. The protein is thought to play a role in the process of inflammation and tissue remodeling. |
| UniProt Protein Function: | CHI3L1: Carbohydrate-binding lectin with a preference for chitin. May play a role in defense against pathogens, or in tissue remodeling. May play an important role in the capacity of cells to respond to and cope with changes in their environment. A genetic variation in CHI3L1 is associated with susceptibility to asthma-related traits type 7 (ASRT7). Asthma-related traits include clinical symptoms of asthma, such as coughing, wheezing and dyspnea, bronchial hyperresponsiveness (BHR) as assessed by methacholine challenge test, serum IgE levels, atopy, and atopic dermatitis. Belongs to the glycosyl hydrolase 18 family. |
| UniProt Protein Details: | Protein type:Secreted, signal peptide; Secreted; Cell adhesion Chromosomal Location of Human Ortholog: 1q32.1 Cellular Component: extracellular space; proteinaceous extracellular matrix; perinuclear region of cytoplasm; endoplasmic reticulum; cytoplasm Molecular Function:extracellular matrix structural constituent; chitin binding; chitinase activity; carbohydrate binding Biological Process: positive regulation of protein kinase B signaling cascade; positive regulation of angiogenesis; apoptosis; response to mechanical stimulus; cartilage development; carbohydrate metabolic process; activation of NF-kappaB-inducing kinase; immune response; chitin catabolic process; inflammatory response; lung development Disease: Schizophrenia; Asthma-related Traits, Susceptibility To, 7 |
| NCBI Summary: | Chitinases catalyze the hydrolysis of chitin, which is an abundant glycopolymer found in insect exoskeletons and fungal cell walls. The glycoside hydrolase 18 family of chitinases includes eight human family members. This gene encodes a glycoprotein member of the glycosyl hydrolase 18 family. The protein lacks chitinase activity and is secreted by activated macrophages, chondrocytes, neutrophils and synovial cells. The protein is thought to play a role in the process of inflammation and tissue remodeling. [provided by RefSeq, Sep 2009] |
| UniProt Code: | P36222 |
| NCBI GenInfo Identifier: | 84028186 |
| NCBI Gene ID: | 1116 |
| NCBI Accession: | P36222.2 |
| UniProt Secondary Accession: | P36222,P30923, Q8IVA4, Q96HI7, B2R7B0, |
| UniProt Related Accession: | P36222 |
| Molecular Weight: | 42,625 Da |
| NCBI Full Name: | Chitinase-3-like protein 1 |
| NCBI Synonym Full Names: | chitinase 3-like 1 (cartilage glycoprotein-39) |
| NCBI Official Symbol: | CHI3L1 |
| NCBI Official Synonym Symbols: | GP39; ASRT7; GP-39; YKL40; CGP-39; YKL-40; YYL-40; HC-gp39; HCGP-3P; hCGP-39 |
| NCBI Protein Information: | chitinase-3-like protein 1; 39 kDa synovial protein; cartilage glycoprotein 39 |
| UniProt Protein Name: | Chitinase-3-like protein 1 |
| UniProt Synonym Protein Names: | 39 kDa synovial protein; Cartilage glycoprotein 39; CGP-39; GP-39; hCGP-39; YKL-40 |
| Protein Family: | Chitinase-3-like protein |
| UniProt Gene Name: | CHI3L1 |
| UniProt Entry Name: | CH3L1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using YKL-40 / CHI3L1 antibody (CAB3166) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded mouse liver using YKL-40 / CHI3L1 antibody (CAB3166) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse spleen using YKL-40 / CHI3L1 antibody (CAB3166) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of Raw264.7 cells using YKL-40 / CHI3L1 antibody (CAB3166) at dilution of 1:100. Blue: DAPI for nuclear staining. |
Related Products
Anti-YKL-40 / CHI3L1 Antibody (CAB1723)
Anti-CHI3L1 Antibody (CAB11510)
