Anti-YBX1 Antibody (CAB7704)[KO Validated]

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SKU:
CAB7704
Product type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
Frequently bought together:

Description

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Antibody Name:Anti-YBX1 Antibody [KO Validated]
Antibody SKU:CAB7704
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 100-200 of human YBX1 (NP_004550.2).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:100 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HepG2
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 100-200 of human YBX1 (NP_004550.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:LRSV GDGE TVEF DVVE GEKG AEAA NVTG PGGV PVQG SKYA ADRN HYRR YPRR RGPP RNYQ QNYQ NSES GEKN EGSE SAPE GQAQ QRRP YRRR RFPP YYMR R
Gene ID:4904
Uniprot:P67809
Cellular Location:Cytoplasm, Cytoplasmic granule, Nucleus, Secreted
Calculated MW:35kDa
Observed MW:49kDa
Synonyms:YBX1, BP-8, CBF-A, CSDA2, CSDB, DBPB, EFI-A, MDR-NF1, NSEP-1, NSEP1, YB-1, YB1
Background:This gene encodes a highly conserved cold shock domain protein that has broad nucleic acid binding properties. The encoded protein functions as both a DNA and RNA binding protein and has been implicated in numerous cellular processes including regulation of transcription and translation, pre-mRNA splicing, DNA reparation and mRNA packaging. This protein is also a component of messenger ribonucleoprotein (mRNP) complexes and may have a role in microRNA processing. This protein can be secreted through non-classical pathways and functions as an extracellular mitogen. Aberrant expression of the gene is associated with cancer proliferation in numerous tissues. This gene may be a prognostic marker for poor outcome and drug resistance in certain cancers. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on multiple chromosomes.
UniProt Protein Function:YB-1: a nuclear protein that binds to splice sites in pre-mRNA and regulates splice site selection. Binds and stabilizes cytoplasmic mRNA. Contributes to the regulation of translation by modulating the interaction between the mRNA and eukaryotic initiation factors CCAAT-containing Y-box of HLA class II genes. Component of cytoplasmic messenger ribonucleoprotein particles (mRNPs). Interacts with AKT1, SFRS9, THOC4, MSH2, XRCC5, WRN and NCL. Can bind to DNA as a homomeric form, (EFI-A)n or as a heteromeric form in association with EFI-B. Homodimer in the presence of ATP. Involved in cisplatin resistance. Seems to be a negative regulatory factor. May play a role in both transcriptional and translational regulation of spermatogenesis. Found at very low levels at day 10 and levels increase at day 15 and persist throughout adulthood.
UniProt Protein Details:

Protein type:Transcription factor; RNA-binding; RNA splicing; RNA processing

Chromosomal Location of Human Ortholog: 1p34

Cellular Component: nucleoplasm; nuclear membrane; intracellular membrane-bound organelle; stress granule; cytoplasm; nucleus; ribonucleoprotein complex; U12-dependent spliceosome

Molecular Function:protein binding; DNA binding; RNA binding; double-stranded DNA binding; transcription factor activity; single-stranded DNA binding

Biological Process: transcription from RNA polymerase II promoter; nuclear mRNA splicing, via spliceosome; negative regulation of striated muscle cell differentiation; regulation of transcription, DNA-dependent; in utero embryonic development; positive regulation of cell division; RNA splicing; positive regulation of transcription from RNA polymerase II promoter; gene expression; negative regulation of transcription from RNA polymerase II promoter

UniProt Code:P67809
NCBI GenInfo Identifier:34098946
NCBI Gene ID:4904
NCBI Accession:NP_004550.2
UniProt Secondary Accession:P67809,P16990, P16991, Q14972, Q15325, Q5FVF0,
UniProt Related Accession:P67809
Molecular Weight:35,924 Da
NCBI Full Name:nuclease-sensitive element-binding protein 1
NCBI Synonym Full Names:Y box binding protein 1
NCBI Official Symbol:YBX1  
NCBI Official Synonym Symbols:YB1; BP-8; CSDB; DBPB; YB-1; CSDA2; NSEP1; NSEP-1; MDR-NF1  
NCBI Protein Information:nuclease-sensitive element-binding protein 1; CBF-A; EFI-A; DNA-binding protein B; Y-box-binding protein 1; Y-box transcription factor; enhancer factor I subunit A; nuclease sensitive element binding protein 1; CCAAT-binding transcription factor I subunit
UniProt Protein Name:Nuclease-sensitive element-binding protein 1
UniProt Synonym Protein Names:CCAAT-binding transcription factor I subunit A; CBF-A; DNA-binding protein B; DBPB; Enhancer factor I subunit A; EFI-A; Y-box transcription factor; Y-box-binding protein 1
Protein Family:Nuclease-sensitive element-binding protein
UniProt Gene Name:YBX1  
UniProt Entry Name:YBOX1_HUMAN
Anti-YBX1 Antibody [KO Validated] (CAB7704)Western blot analysis of extracts of HepG2 cells, using YBX1 antibody (CAB7704) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Anti-YBX1 Antibody [KO Validated] (CAB7704)Western blot analysis of extracts from normal (control) and YBX1 knockout (KO) HeLa cells, using YBX1 antibody (CAB7704) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
Anti-YBX1 Antibody [KO Validated] (CAB7704)Immunohistochemistry of paraffin-embedded rat testis using YBX1 antibody (CAB7704) at dilution of 1:100 (40x lens).
Anti-YBX1 Antibody [KO Validated] (CAB7704)Immunohistochemistry of paraffin-embedded mouse testis using YBX1 antibody (CAB7704) at dilution of 1:100 (40x lens).
Anti-YBX1 Antibody [KO Validated] (CAB7704)Immunohistochemistry of paraffin-embedded human colon carcinoma using YBX1 antibody (CAB7704) at dilution of 1:100 (40x lens).

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