Anti-WNT3A Antibody (CAB0642)
- SKU:
- CAB0642
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-WNT3A Antibody |
| Antibody SKU: | CAB0642 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-352 of human WNT3A (NP_149122.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U2OS, A-549, PC-3, Raji, 293T, Mouse lung, Mouse testis, Rat lung, Rat testis |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-352 of human WNT3A (NP_149122.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAPL GYFL LLCS LKQA LGSY PIWW SLAV GPQY SSLG SQPI LCAS IPGL VPKQ LRFC RNYV EIMP SVAE GIKI GIQE CQHQ FRGR RWNC TTVH DSLA IFGP VLDK ATRE SAFV HAIA SAGV AFAV TRSC AEGT AAIC GCSS RHQG SPGK GWKW GGCS EDIE FGGM VSRE FADA RENR PDAR SAMN RHNN EAGR QAIA SHMH LKCK CHGL SGSC EVKT CWWS QPDF RAIG DFLK DKYD SASE MVVE KHRE SRGW VETL RPRY TYFK VPTE RDLV YYEA SPNF CEPN PETG SFGT RDRT CNVS SHGI DGCD LLCC GRGH NARA ERRR EKCR CVFH WCCY VSCQ ECTR VYDV HTCK |
| Gene ID: | 89780 |
| Uniprot: | P56704 |
| Cellular Location: | Secreted, extracellular matrix, extracellular space |
| Calculated MW: | 39kDa/42kDa |
| Observed MW: | 39kDa |
| Synonyms: | WNT3A |
| Background: | The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 96% amino acid identity to mouse Wnt3A protein, and 84% to human WNT3 protein, another WNT gene product. This gene is clustered with WNT14 gene, another family member, in chromosome 1q42 region. |
| UniProt Protein Function: | WNT3A: Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube. Interacts with PORCN. Interacts with APCDD1 and WLS. Component of the Wnt-Fzd-LRP5-LRP6 signaling complex that contains a WNT protein, a FZD protein and LRP5 or LRP6. Interacts directly in the complex with LRP6. Moderately expressed in placenta and at low levels in adult lung, spleen, and prostate. Belongs to the Wnt family. |
| UniProt Protein Details: | Protein type:Secreted; Secreted, signal peptide Chromosomal Location of Human Ortholog: 1q42 Cellular Component: proteinaceous extracellular matrix; extracellular space; cell surface; endoplasmic reticulum lumen; early endosome membrane; Golgi lumen; plasma membrane; extracellular region Molecular Function:protein domain specific binding; protein binding; frizzled binding; transcription coactivator activity; receptor agonist activity; frizzled-2 binding Biological Process: positive regulation of mesodermal cell fate specification; axon guidance; extracellular matrix organization and biogenesis; positive regulation of protein binding; positive regulation of transcription, DNA-dependent; cell proliferation in forebrain; positive regulation of receptor internalization; positive regulation of caspase activity; Wnt receptor signaling pathway through beta-catenin; positive regulation of collateral sprouting in the absence of injury; palate development; negative regulation of axon extension involved in axon guidance; Wnt receptor signaling pathway in forebrain neuroblast division; negative regulation of neurogenesis; neuron differentiation; mammary gland development; positive regulation of cell proliferation; positive regulation of B cell proliferation; hemopoiesis; heart looping; dorsoventral neural tube patterning; inner ear morphogenesis; in utero embryonic development; hippocampus development; negative regulation of fat cell differentiation; positive regulation of peptidyl-serine phosphorylation; osteoblast differentiation; paraxial mesodermal cell fate commitment; signalosome assembly; spinal cord association neuron differentiation; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation |
| NCBI Summary: | The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 96% amino acid identity to mouse Wnt3A protein, and 84% to human WNT3 protein, another WNT gene product. This gene is clustered with WNT14 gene, another family member, in chromosome 1q42 region. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P56704 |
| NCBI GenInfo Identifier: | 20532424 |
| NCBI Gene ID: | 89780 |
| NCBI Accession: | P56704.2 |
| UniProt Secondary Accession: | P56704,Q3SY79, Q3SY80, Q969P2, |
| UniProt Related Accession: | P56704 |
| Molecular Weight: | 39kDa |
| NCBI Full Name: | Protein Wnt-3a |
| NCBI Synonym Full Names: | wingless-type MMTV integration site family, member 3A |
| NCBI Official Symbol: | WNT3A |
| NCBI Protein Information: | protein Wnt-3a |
| UniProt Protein Name: | Protein Wnt-3a |
| UniProt Gene Name: | WNT3A |
| UniProt Entry Name: | WNT3A_HUMAN |
 | Western blot analysis of extracts of various cell lines, using WNT3A antibody (CAB0642) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3min. |
 | Immunohistochemistry of paraffin-embedded Human lung using WNT3A antibody (CAB0642) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of human placenta cells using WNT3A antibody (CAB0642) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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