Anti-WNT10B Antibody (CAB16717)
- SKU:
- CAB16717
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Frequently bought together:
Description
| Antibody Name: | Anti-WNT10B Antibody |
| Antibody SKU: | CAB16717 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant Protein of human WNT10B |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa |
| Immunogen: | Recombinant Protein of human WNT10B |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 7480 |
| Uniprot: | O00744 |
| Cellular Location: | |
| Calculated MW: | |
| Observed MW: | 43kDa |
| Synonyms: | WNT10B, SHFM6, STHAG8, WNT-12 |
| Background: | The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It may be involved in breast cancer, and its protein signaling is likely a molecular switch that governs adipogenesis. This protein is 96% identical to the mouse Wnt10b protein at the amino acid level. This gene is clustered with another family member, WNT1, in the chromosome 12q13 region. |
| UniProt Protein Function: | WNT10B: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. Defects in WNT10B are the cause of split-hand/foot malformation type 6 (SHFM6). SHFM is a limb malformation involving the central rays of the autopod and presenting with syndactyly, median clefts of the hands and feet, and aplasia and/or hypoplasia of the phalanges, metacarpals, and metatarsals. SHFM6 is a autosomal recessive disorder. Belongs to the Wnt family. |
| UniProt Protein Details: | Protein type:Secreted, signal peptide; Secreted Chromosomal Location of Human Ortholog: 12q13 Cellular Component: extracellular region; extracellular space Molecular Function:frizzled binding; receptor agonist activity Biological Process: cell fate commitment; chondrocyte differentiation; negative regulation of fat cell differentiation; neuron differentiation; positive regulation of apoptosis; protein stabilization; Wnt receptor signaling pathway; Wnt receptor signaling pathway through beta-catenin Disease: Split-hand/foot Malformation 6 |
| NCBI Summary: | The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It may be involved in breast cancer, and its protein signaling is likely a molecular switch that governs adipogenesis. This protein is 96% identical to the mouse Wnt10b protein at the amino acid level. This gene is clustered with another family member, WNT1, in the chromosome 12q13 region. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O00744 |
| NCBI GenInfo Identifier: | 20532419 |
| NCBI Gene ID: | 7480 |
| NCBI Accession: | O00744.2 |
| UniProt Secondary Accession: | O00744,O00747, Q4VAJ4, Q4VAJ5, Q8WZ97, B2R7A5, |
| UniProt Related Accession: | O00744 |
| Molecular Weight: | 20,483 Da |
| NCBI Full Name: | Protein Wnt-10b |
| NCBI Synonym Full Names: | Wnt family member 10B |
| NCBI Official Symbol: | WNT10B |
| NCBI Official Synonym Symbols: | SHFM6; WNT-12 |
| NCBI Protein Information: | protein Wnt-10b |
| UniProt Protein Name: | Protein Wnt-10b |
| UniProt Synonym Protein Names: | Protein Wnt-12 |
| UniProt Gene Name: | WNT10B |
| UniProt Entry Name: | WN10B_HUMAN |
 | Western blot analysis of extracts of HeLa cells, using WNT10B antibody (CAB16717) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunohistochemistry of paraffin-embedded rat ovary using WNT10B antibody (CAB16717) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human colon carcinoma using WNT10B antibody (CAB16717) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of C6 cells using WNT10B antibody (CAB16717) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of L929 cells using WNT10B antibody (CAB16717) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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