Anti-WNK3 항체 (CAB15507)

Antibody Name:	Anti-WNK3 Antibody
Antibody SKU:	CAB15507
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 500-600 of human WNK3 (NP_065973.2).

Application:	WB
Recommended Dilution:	WB 1:200 - 1:2000
Reactivity:	Mouse
Positive Samples:	Mouse brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 500-600 of human WNK3 (NP_065973.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	AGCL EERR DSQC KSMG NVFP QPQN TTLP LAPA QQTG AECE ETEV DQHV RQQL LQRK PQQH CSSV TGDN LSEA GAAS VIHS DTSS QPSV AYSS NQTM GSQM V
Gene ID:	65267
Uniprot:	Q9BYP7
Cellular Location:	Cytoplasm
Calculated MW:	191kDa/192kDa/197kDa/198kDa
Observed MW:	198kDa

Synonyms:

WNK3, PRKWNK3

Background:

This gene encodes a protein belonging to the 'with no lysine' family of serine-threonine protein kinases. These family members lack the catalytic lysine in subdomain II, and instead have a conserved lysine in subdomain I. This family member functions as a positive regulator of the transcellular Ca2+ transport pathway, and it plays a role in the increase of cell survival in a caspase-3-dependent pathway. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	WNK3: Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Phosphorylates WNK4. Regulates the phosphorylation of SLC12A1 and SLC12A2. Increases Ca(2+) influx mediated by TRPV5 and TRPV6 by enhancing their membrane expression level via a kinase-dependent pathway. Inhibits the activity of KCNJ1 by decreasing its expression at the cell membrane in a non-catalytic manner. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WNK subfamily. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Protein kinase, Other; EC 2.7.11.1; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Other group; Wnk family Chromosomal Location of Human Ortholog: Xp11.22 Cellular Component: adherens junction; cytoplasm; cytosol; tight junction Molecular Function:ATP binding; chloride channel inhibitor activity; protein binding; protein kinase activity; protein serine/threonine kinase activity Biological Process: negative regulation of apoptosis; positive regulation of calcium ion transport; positive regulation of ion transmembrane transporter activity; protein amino acid autophosphorylation; protein amino acid phosphorylation
NCBI Summary:	This gene encodes a protein belonging to the 'with no lysine' family of serine-threonine protein kinases. These family members lack the catalytic lysine in subdomain II, and instead have a conserved lysine in subdomain I. This family member functions as a positive regulator of the transcellular Ca2+ transport pathway, and it plays a role in the increase of cell survival in a caspase-3-dependent pathway. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2010]
UniProt Code:	Q9BYP7
NCBI GenInfo Identifier:	353526307
NCBI Gene ID:	65267
NCBI Accession:	Q9BYP7.3
UniProt Secondary Accession:	Q9BYP7,Q5JRC1, Q6JP76, Q8TCX6, Q9HCK6, B1AKG2,
UniProt Related Accession:	Q9BYP7
Molecular Weight:	192,875 Da
NCBI Full Name:	Serine/threonine-protein kinase WNK3
NCBI Synonym Full Names:	WNK lysine deficient protein kinase 3
NCBI Official Symbol:	WNK3
NCBI Official Synonym Symbols:	PRKWNK3
NCBI Protein Information:	serine/threonine-protein kinase WNK3
UniProt Protein Name:	Serine/threonine-protein kinase WNK3
UniProt Synonym Protein Names:	Protein kinase lysine-deficient 3; Protein kinase with no lysine 3
Protein Family:	Serine/threonine-protein kinase
UniProt Gene Name:	WNK3
UniProt Entry Name:	WNK3_HUMAN

Western blot analysis of extracts of mouse brain, using WNK3 antibody (CAB15507) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)