Anti-WIPI1 Antibody (CAB7528)
- SKU:
- CAB7528
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Autophagy
Frequently bought together:
Description
| Antibody Name: | Anti-WIPI1 Antibody |
| Antibody SKU: | CAB7528 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 187-446 of human WIPI1 (NP_060453.3). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | SW480, BxPC-3, Mouse eye, Rat brain, Rat eye |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 187-446 of human WIPI1 (NP_060453.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | GTLA AITF NASG SKLA SASE KGTV IRVF SVPD GQKL YEFR RGMK RYVT ISSL VFSM DSQF LCAS SNTE TVHI FKLE QVTN SRPE EPST WSGY MGKM FMAA TNYL PTQV SDMM HQDR AFAT ARLN FSGQ RNIC TLST IQKL PRLL VASS SGHL YMYN LDPQ DGGE CVLI KTHS LLGS GTTE ENKE NDLR PSLP QSYA ATVA RPSA SSAS TVPG YSED GGAL RGEV IPEH EFAT GPVC LDDE NEFP PIIL CRGN QKGK TKQS |
| Gene ID: | 55062 |
| Uniprot: | Q5MNZ9 |
| Cellular Location: | Cytoplasm, Cytoplasmic vesicle, Endosome, Golgi apparatus, Peripheral membrane protein, Preautophagosomal structure membrane, clathrin-coated vesicle, cytoskeleton, trans-Golgi network |
| Calculated MW: | 48kDa |
| Observed MW: | 46kDa |
| Synonyms: | WIPI1, ATG18, ATG18A, WIPI49 |
| Background: | This gene encodes a WD40 repeat protein. Members of the WD40 repeat family are key components of many essential biologic functions. They regulate the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. Members of the WIPI subfamily of WD40 repeat proteins have a 7-bladed propeller structure and contain a conserved motif for interaction with phospholipids. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | ATG18: May play a role in autophagy. May regulate the trafficking of proteins involved in the mannose-6-phosphate receptor (MPR) recycling pathway. Probably interacts with androgen (AR) and the estrogen receptor (ER). Binds PtdIns3P and to a lesser extent, PtdIns3,5P2 and PtdIns5P in vitro. Interaction with PtdIns3P is required for recruitment to membranes. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Autophagy Chromosomal Location of Human Ortholog: 17q24.2 Cellular Component: cytoplasm; cytosol; endosome membrane; extrinsic to membrane; Golgi membrane; pre-autophagosomal structure membrane; trans-Golgi network Molecular Function:androgen receptor binding; estrogen receptor binding; phosphatidylinositol 3-phosphate binding; receptor binding Biological Process: autophagic vacuole formation; autophagy; macroautophagy; mitochondrion degradation; protein amino acid lipidation; vesicle targeting, trans-Golgi to endosome |
| NCBI Summary: | This gene encodes a WD40 repeat protein. Members of the WD40 repeat family are key components of many essential biologic functions. They regulate the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. Members of the WIPI subfamily of WD40 repeat proteins have a 7-bladed propeller structure and contain a conserved motif for interaction with phospholipids. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016] |
| UniProt Code: | Q5MNZ9 |
| NCBI GenInfo Identifier: | 146291100 |
| NCBI Gene ID: | 55062 |
| NCBI Accession: | Q5MNZ9.3 |
| UniProt Secondary Accession: | Q5MNZ9,Q8IXM5, Q9NWF8, |
| UniProt Related Accession: | Q5MNZ9 |
| Molecular Weight: | 48,202 Da |
| NCBI Full Name: | WD repeat domain phosphoinositide-interacting protein 1 |
| NCBI Synonym Full Names: | WD repeat domain, phosphoinositide interacting 1 |
| NCBI Official Symbol: | WIPI1 |
| NCBI Official Synonym Symbols: | ATG18; ATG18A; WIPI49 |
| NCBI Protein Information: | WD repeat domain phosphoinositide-interacting protein 1 |
| UniProt Protein Name: | WD repeat domain phosphoinositide-interacting protein 1 |
| UniProt Synonym Protein Names: | Atg18 protein homolog; WD40 repeat protein interacting with phosphoinositides of 49 kDa; WIPI 49 kDa |
| Protein Family: | WD repeat domain phosphoinositide-interacting protein |
| UniProt Gene Name: | WIPI1 |
| UniProt Entry Name: | WIPI1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using WIPI1 antibody (CAB7528) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded human brain cancer using WIPI1 Antibody (CAB7528) at dilution of 1:100 (40x lens). |
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