Anti-WDR81 Antibody (CAB12780)
- SKU:
- CAB12780
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-WDR81 Antibody |
| Antibody SKU: | CAB12780 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 360-600 of human WDR81 (NP_689561.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 360-600 of human WDR81 (NP_689561.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | RIGQ EMVQ QHLS EPVA TFFQ VFSQ LHEL RQQD LKLD PAGR GEGQ LPQV VFSD GQQR PVDP ALLD ELQK VFTL EMAY TIYV PFSC LLGD IIRK IIPN HELV GELA ALYL ESIS PSSR NPAS VEPT MPGT GPEW DPHG GGCP QDDG HSGT FGSV LVGN RIQI PNDS RPEN PGPL GPIS GVGG GGLG SGSD DNAL KQEL PRSV HGLS GNWL AYWQ YEIG VSQQ DAHF HFHQ IRLQ SFPG HSGA V |
| Gene ID: | 124997 |
| Uniprot: | Q562E7 |
| Cellular Location: | Mitochondrion |
| Calculated MW: | 74kDa/77kDa/79kDa/95kDa/197kDa/211kDa |
| Observed MW: | 260kDa |
| Synonyms: | WDR81, CAMRQ2, PPP1R166, SORF-2 |
| Background: | This gene encodes a multi-domain transmembrane protein, which is predominantly expressed in the brain. Mutations in this gene are associated with autosomal recessive cerebellar ataxia, mental retardation, and dysequilibrium syndrome-2. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | WDR81: 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Unknown function Chromosomal Location of Human Ortholog: 17p13.3 Disease: Cerebellar Ataxia, Mental Retardation, And Dysequilibrium Syndrome 2 |
| NCBI Summary: | This gene encodes a multi-domain transmembrane protein, which is predominantly expressed in the brain. Mutations in this gene are associated with autosomal recessive cerebellar ataxia, mental retardation, and dysequilibrium syndrome-2. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2012] |
| UniProt Code: | Q562E7 |
| NCBI GenInfo Identifier: | 403314383 |
| NCBI Gene ID: | 124997 |
| NCBI Accession: | Q562E7.2 |
| UniProt Secondary Accession: | Q562E7,Q24JP6, Q8N277, Q8N3F3, Q8TEL1, B3KW16, B3KXU1 B7Z579, E9PHG7, |
| UniProt Related Accession: | Q562E7 |
| Molecular Weight: | 212kDa |
| NCBI Full Name: | WD repeat-containing protein 81 |
| NCBI Synonym Full Names: | WD repeat domain 81 |
| NCBI Official Symbol: | WDR81 |
| NCBI Official Synonym Symbols: | CAMRQ2; SORF-2; PPP1R166 |
| NCBI Protein Information: | WD repeat-containing protein 81 |
| UniProt Protein Name: | WD repeat-containing protein 81 |
| Protein Family: | WD repeat-containing protein |
| UniProt Gene Name: | WDR81 |
 | Western blot analysis of extracts of Mouse brain, using WDR81 antibody (CAB12780) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of HeLa cells using WDR81 antibody (CAB12780) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of NIH/3T3 cells using WDR81 antibody (CAB12780) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of PC12 cells using WDR81 antibody (CAB12780) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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