Anti-WASF3 Antibody (CAB16274)
- SKU:
- CAB16274
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-WASF3 Antibody |
| Antibody SKU: | CAB16274 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 200-300 of human WASF3 (NP_001278894.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:100 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse brain, Rat brain |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 200-300 of human WASF3 (NP_001278894.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | RTRK EEWE RRKM GIEF MSDA KKLE QAGS AKED RVPS GSHA SDVT DYSY PATP NHSL HPQP VTPS YAAG DVPP HGPA SQAA EHEY RPPS ASAR HMAL NRPQ Q |
| Gene ID: | 10810 |
| Uniprot: | Q9UPY6 |
| Cellular Location: | |
| Calculated MW: | 55kDa |
| Observed MW: | 55kDa |
| Synonyms: | WASF3, Brush-1, SCAR3, WAVE3 |
| Background: | This gene encodes a member of the Wiskott-Aldrich syndrome protein family. The gene product is a protein that forms a multiprotein complex that links receptor kinases and actin. Binding to actin occurs through a C-terminal verprolin homology domain in all family members. The multiprotein complex serves to tranduce signals that involve changes in cell shape, motility or function. A pseudogene of this gene have been defined on chromosome 6. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | WASF3: Downstream effector molecules involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape. Binds actin and the Arp2/3 complex. Expressed in ovary and brain. Belongs to the SCAR/WAVE family. |
| UniProt Protein Details: | Protein type:Actin-binding; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 13q12 Cellular Component: cytoskeleton; lamellipodium; cytoplasm Molecular Function:actin binding Biological Process: positive regulation of myelination; lamellipodium biogenesis; regulation of cell shape; actin filament polymerization; cytoskeleton organization and biogenesis; protein complex assembly; oligodendrocyte development |
| NCBI Summary: | This gene encodes a member of the Wiskott-Aldrich syndrome protein family. The gene product is a protein that forms a multiprotein complex that links receptor kinases and actin. Binding to actin occurs through a C-terminal verprolin homology domain in all family members. The multiprotein complex serves to tranduce signals that involve changes in cell shape, motility or function. A pseudogene of this gene have been defined on chromosome 6. Alternative splicing results in multiple transcript variants [provided by RefSeq, May 2014] |
| UniProt Code: | Q9UPY6 |
| NCBI GenInfo Identifier: | 13699803 |
| NCBI Gene ID: | 10810 |
| NCBI Accession: | NP_006637.2 |
| UniProt Secondary Accession: | Q9UPY6,O94974, Q86VQ2, |
| UniProt Related Accession: | Q9UPY6 |
| Molecular Weight: | 55,293 Da |
| NCBI Full Name: | wiskott-Aldrich syndrome protein family member 3 isoform 1 |
| NCBI Synonym Full Names: | WAS protein family, member 3 |
| NCBI Official Symbol: | WASF3 |
| NCBI Official Synonym Symbols: | SCAR3; WAVE3; Brush-1 |
| NCBI Protein Information: | wiskott-Aldrich syndrome protein family member 3; protein WAVE-3; WASP family Verprolin-homologous protein 3; verprolin homology domain-containing protein 3 |
| UniProt Protein Name: | Wiskott-Aldrich syndrome protein family member 3 |
| UniProt Synonym Protein Names: | Protein WAVE-3; Verprolin homology domain-containing protein 3 |
| Protein Family: | Wiskott-Aldrich syndrome protein family |
| UniProt Gene Name: | WASF3 |
| UniProt Entry Name: | WASF3_HUMAN |
 | Western blot analysis of extracts of various cell lines, using WASF3 antibody (CAB16274) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s. |
 | Immunohistochemistry of paraffin-embedded human lung using WASF3 antibody (CAB16274) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human breast cancer using WASF3 antibody (CAB16274) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human stomach using WASF3 antibody (CAB16274) at dilution of 1:100 (40x lens). |
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