Anti-WARS Antibody (CAB5758)
- SKU:
- CAB5758
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cardiovascular
Frequently bought together:
Description
| Antibody Name: | Anti-WARS Antibody |
| Antibody SKU: | CAB5758 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human WARS (NP_776049.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:10 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | MCF7, SW480, 293T, HeLa, mouse brain, mouse lung, rat brain, HL-60, HepG2 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human WARS (NP_776049.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MPNS EPAS LLEL FNSI ATQG ELVR SLKA GNAS KDEI DSAV KMLV SLKM SYKA AAGE DYKA DCPP GNPA PTSN HGPD ATEA EEDF VDPW TVQT SSAK GIDY DKLI VRFG SSKI DKEL INRI ERAT GQRP HHFL RRGI FFSH RDMN QVLD AYEN KKPF YLYT GRGP SSEA MHVG HLIP FIFT KWLQ DVFN VPLV IQMT DDEK YLWK DLTL DQAY SYAV ENAK DIIA CGFD INKT FIFS DLDY MGMS SGFY KNVV KIQK HVTF NQVK GIFG FT |
| Gene ID: | 7453 |
| Uniprot: | P23381 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 48kDa/53kDa |
| Observed MW: | 53-55kDa |
| Synonyms: | WARS, GAMMA-2, IFI53, IFP53 |
| Background: | Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of tryptophanyl-tRNA synthetase exist, a cytoplasmic form, named WARS, and a mitochondrial form, named WARS2. Tryptophanyl-tRNA synthetase (WARS) catalyzes the aminoacylation of tRNA(trp) with tryptophan and is induced by interferon. Tryptophanyl-tRNA synthetase belongs to the class I tRNA synthetase family. Four transcript variants encoding two different isoforms have been found for this gene. |
| UniProt Protein Function: | WARS: Isoform 1, isoform 2 and T1-TrpRS have aminoacylation activity while T2-TrpRS lacks it. Isoform 2, T1-TrpRS and T2-TrpRS possess angiostatic activity whereas isoform 1 lacks it. T2-TrpRS inhibits fluid shear stress-activated responses of endothelial cells. Regulates ERK, Akt, and eNOS activation pathways that are associated with angiogenesis, cytoskeletal reorganization and shear stress-responsive gene expression. Belongs to the class-I aminoacyl-tRNA synthetase family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Translation; Amino Acid Metabolism - tryptophan; Ligase; EC 6.1.1.2 Chromosomal Location of Human Ortholog: 14q32.31 Cellular Component: cytoplasm; nucleus; cytosol Molecular Function:protein binding; tryptophan-tRNA ligase activity; ATP binding Biological Process: negative regulation of cell proliferation; tRNA aminoacylation for protein translation; tryptophanyl-tRNA aminoacylation; translation; gene expression; angiogenesis; regulation of angiogenesis |
| NCBI Summary: | Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of tryptophanyl-tRNA synthetase exist, a cytoplasmic form, named WARS, and a mitochondrial form, named WARS2. Tryptophanyl-tRNA synthetase (WARS) catalyzes the aminoacylation of tRNA(trp) with tryptophan and is induced by interferon. Tryptophanyl-tRNA synthetase belongs to the class I tRNA synthetase family. Four transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P23381 |
| NCBI GenInfo Identifier: | 135191 |
| NCBI Gene ID: | 7453 |
| NCBI Accession: | P23381.2 |
| UniProt Secondary Accession: | P23381,P78535, Q502Y0, Q53XB6, Q9UDI5, Q9UDL3, A6NGN1 A6NID3, |
| UniProt Related Accession: | P23381 |
| Molecular Weight: | 471 |
| NCBI Full Name: | Tryptophan--tRNA ligase, cytoplasmic |
| NCBI Synonym Full Names: | tryptophanyl-tRNA synthetase |
| NCBI Official Symbol: | WARS |
| NCBI Official Synonym Symbols: | IFI53; IFP53; GAMMA-2 |
| NCBI Protein Information: | tryptophan--tRNA ligase, cytoplasmic; hWRS; trpRS; interferon-induced protein 53; tryptophan tRNA ligase 1, cytoplasmic |
| UniProt Protein Name: | Tryptophan--tRNA ligase, cytoplasmic |
| UniProt Synonym Protein Names: | Interferon-induced protein 53; IFP53; Tryptophanyl-tRNA synthetase; TrpRS; hWRS |
| Protein Family: | Tryptophan--tRNA ligase |
| UniProt Gene Name: | WARS |
| UniProt Entry Name: | SYWC_HUMAN |
 | Western blot analysis of extracts of various cell lines, using WARS antibody (CAB5758) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
 | Immunofluorescence analysis of U2OS cells using WARS antibody (CAB5758). Blue: DAPI for nuclear staining. |
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