Anti-VPS39 Antibody (CAB13082)
- SKU:
- CAB13082
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Autophagy
Frequently bought together:
Description
| Antibody Name: | Anti-VPS39 Antibody |
| Antibody SKU: | CAB13082 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 606-875 of human VPS39 (NP_056104.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | U-87MG, 293T, A-549 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 606-875 of human VPS39 (NP_056104.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | RFHN CLIQ LYCE KVQG LMKE YLLS FPAG KTPV PAGE EEGE LGEY RQKL LMFL EISS YYDP GRLI CDFP FDGL LEER ALLL GRMG KHEQ ALFI YVHI LKDT RMAE EYCH KHYD RNKD GNKD VYLS LLRM YLSP PSIH CLGP IKLE LLEP KANL QAAL QVLE LHHS KLDT TKAL NLLP ANTQ INDI RIFL EKVL EENA QKKR FNQV LKNL LHAE FLRV QEER ILHQ QVKC IITE EKVC MVCK KKIG NSAF ARYP NGVV VHYF CSKE VNPA DT |
| Gene ID: | 23339 |
| Uniprot: | Q96JC1 |
| Cellular Location: | Cytoplasm, Late endosome, Late endosome membrane, Lysosome, Lysosome membrane, Peripheral membrane protein |
| Calculated MW: | 100kDa/101kDa |
| Observed MW: | 102kDa |
| Synonyms: | VPS39, TLP, VAM6, hVam6p |
| Background: | This gene encodes a protein that may promote clustering and fusion of late endosomes and lysosomes. The protein may also act as an adaptor protein that modulates the transforming growth factor-beta response by coupling the transforming growth factor-beta receptor complex to the Smad pathway. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | VPS39: May play a role in clustering and fusion of late endosomes and lysosomes. Regulator of TGF-beta/activin signaling, inhibiting SMAD3- and activating SMAD2-dependent transcription. Acts by interfering with SMAD3/SMAD4 complex formation, this would lead to inhibition of SMAD3-dependent transcription and relieve SMAD3 inhibition of SMAD2-dependent promoters, thus increasing SMAD2-dependent transcription. Does not affect TGF-beta-induced SMAD2 or SMAD3 phosphorylation, nor SMAD2/SMAD4 complex formation. Belongs to the VAM6/VPS39 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Membrane protein, peripheral; G protein regulator, misc. Chromosomal Location of Human Ortholog: 15q15.1 Cellular Component: lysosomal membrane; late endosome membrane Biological Process: vesicle-mediated transport; intracellular protein transport; regulation of catalytic activity |
| NCBI Summary: | This gene encodes a protein that may promote clustering and fusion of late endosomes and lysosomes. The protein may also act as an adaptor protein that modulates the transforming growth factor-beta response by coupling the transforming growth factor-beta receptor complex to the Smad pathway. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014] |
| UniProt Code: | Q96JC1 |
| NCBI GenInfo Identifier: | 33320680 |
| NCBI Gene ID: | 23339 |
| NCBI Accession: | AAQ05978 |
| UniProt Related Accession: | Q96JC1 |
| Molecular Weight: | 100kDa; 101kDa |
| NCBI Full Name: | VPS39 |
| NCBI Synonym Full Names: | VPS39 subunit of HOPS complex |
| NCBI Official Symbol: | VPS39 |
| NCBI Official Synonym Symbols: | TLP; VAM6; hVam6p |
| NCBI Protein Information: | vam6/Vps39-like protein |
| UniProt Protein Name: | Vam6/Vps39-like protein |
| UniProt Synonym Protein Names: | TRAP1-like protein; hVam6p |
| Protein Family: | Vam6/Vps39-like protein |
| UniProt Gene Name: | VPS39 |
| UniProt Entry Name: | VPS39_HUMAN |
 | Western blot analysis of extracts of various cell lines, using VPS39 antibody (CAB13082) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
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