Anti-VPS28 Antibody (CAB9104)
- SKU:
- CAB9104
- Product type:
- Antibody
- Application:
- WB
- Application:
- IF
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-VPS28 Antibody |
| Antibody SKU: | CAB9104 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-221 of human VPS28 (NP_057292.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | A-549, U-251MG, 293T, HepG2, Mouse brain, Mouse kidney, Mouse liver, Mouse spleen, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-221 of human VPS28 (NP_057292.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MFHG IPAT PGIG APGN KPEL YEEV KLYK NARE REKY DNMA ELFA VVKT MQAL EKAY IKDC VSPS EYTA ACSR LLVQ YKAA FRQV QGSE ISSI DEFC RKFR LDCP LAME RIKE DRPI TIKD DKGN LNRC IADV VSLF ITVM DKLR LEIR AMDE IQPD LREL METM HRMS HLPP DFEG RQTV SQWL QTLS GMSA SDEL DDSQ VRQM LFDL ESAY NAFN RFLH A |
| Gene ID: | 51160 |
| Uniprot: | Q9UK41 |
| Cellular Location: | Cell membrane, Late endosome membrane, Peripheral membrane protein |
| Calculated MW: | 25kDa/26kDa |
| Observed MW: | 25kDa |
| Synonyms: | VPS28 |
| Background: | This gene encodes a protein subunit of the ESCRT-I complex (endosomal complexes required for transport), which functions in the transport and sorting of proteins into subcellular vesicles. This complex can also be hijacked to facilitate the budding of enveloped viruses from the cell membrane. Alternative splicing results in multiple transcript variants encoding different isoforms. |
| UniProt Protein Function: | Component of the ESCRT-I complex, a regulator of vesicular trafficking process. |
| NCBI Summary: | This gene encodes a protein subunit of the ESCRT-I complex (endosomal complexes required for transport), which functions in the transport and sorting of proteins into subcellular vesicles. This complex can also be hijacked to facilitate the budding of enveloped viruses from the cell membrane. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013] |
| UniProt Code: | Q9UK41 |
| NCBI GenInfo Identifier: | 7705885 |
| NCBI Gene ID: | 51160 |
| NCBI Accession: | NP_057292 |
| UniProt Secondary Accession: | Q9UK41,Q86VK0, |
| UniProt Related Accession: | Q9UK41 |
| Molecular Weight: | 25.4 kDa (221aa), confirmed by MALDI-TOF. |
| NCBI Full Name: | vacuolar protein sorting-associated protein 28 homolog isoform 1 |
| NCBI Synonym Full Names: | VPS28, ESCRT-I subunit |
| NCBI Official Symbol: | VPS28 |
| NCBI Protein Information: | vacuolar protein sorting-associated protein 28 homolog |
| UniProt Protein Name: | Vacuolar protein sorting-associated protein 28 homolog |
| UniProt Synonym Protein Names: | ESCRT-I complex subunit VPS28 |
| Protein Family: | Vacuolar protein sorting-associated protein |
| UniProt Gene Name: | VPS28 |
 | Western blot analysis of extracts of various cell lines, using VPS28 antibody (CAB9104) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
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