Anti-VPS16 항체 (CAB15888)

Antibody Name:	Anti-VPS16 Antibody
Antibody SKU:	CAB15888
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 540-839 of human VPS16 (NP_072097.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-549, Mouse brain, Mouse lung, Rat pancreas

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 540-839 of human VPS16 (NP_072097.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	EYEP RSGE QVPL LLKM KRSK LALS KAIE SGDT DLVF TVLL HLKN ELNR GDFF MTLR NQPM ALSL YRQF CKHQ ELET LKDL YNQD DNHQ ELGS FHIR ASYA AEER IEGR VAAL QTAA DAFY KAKN EFAA KATE DQMR LLRL QRRL EDEL GGQF LDLS LHDT VTTL ILGG HNKR AEQL ARDF RIPD KRLW WLKL TALA DLED WEEL EKFS KSKK SPIG YLPF VEIC MKQH NKYE AKKY ASRV GPEQ KVKA LLLV GDVA QAAD VAIE HRNE AELS LVLS HCTG ATDG ATAD KIQR ARAQ AQKK
Gene ID:	64601
Uniprot:	Q9H269
Cellular Location:	Cytoplasmic side, Cytoplasmic vesicle, Early endosome, Late endosome membrane, Lysosome membrane, Peripheral membrane protein, autophagosome, clathrin-coated vesicle
Calculated MW:	78kDa/94kDa
Observed MW:	95kDa

Synonyms:

VPS16, hVPS16

Background:

Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene encodes the human homolog of yeast class C Vps16 protein. The mammalian class C Vps proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway. Two transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	Plays a role in vesicle-mediated protein trafficking to lysosomal compartments including the endocytic membrane transport and autophagic pathways. Believed to act as a core component of the putative HOPS and CORVET endosomal tethering complexes which are proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion. The HOPS complex is proposed to be recruited to Rab7 on the late endosomal membrane and to regulate late endocytic, phagocytic and autophagic traffic towards lysosomes. The CORVET complex is proposed to function as a Rab5 effector to mediate early endosome fusion probably in specific endosome subpopulations (PubMed:11382755, PubMed:23351085, PubMed:24554770, PubMed:25266290, PubMed:25783203). Required for recruitment of VPS33A to the HOPS complex (PubMed:23901104). Required for fusion of endosomes and autophagosomes with lysosomes; the function is dependent on its association with VPS33A but not VPS33B (PubMed:25783203). The function in autophagosome-lysosome fusion implicates STX17 but not UVRAG (PubMed:24554770).
NCBI Summary:	Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene encodes the human homolog of yeast class C Vps16 protein. The mammalian class C Vps proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2009]
UniProt Code:	Q9H269
NCBI GenInfo Identifier:	17978479
NCBI Gene ID:	64601
NCBI Accession:	NP_072097.2
UniProt Secondary Accession:	Q9H269,Q5JUB1, Q8WU31, Q96EE7, Q96N92, Q9H1Q4, Q9H1Q5
UniProt Related Accession:	Q9H269
Molecular Weight:	78,300 Da
NCBI Full Name:	vacuolar protein sorting-associated protein 16 homolog isoform 1
NCBI Synonym Full Names:	VPS16, CORVET/HOPS core subunit
NCBI Official Symbol:	VPS16
NCBI Official Synonym Symbols:	hVPS16
NCBI Protein Information:	vacuolar protein sorting-associated protein 16 homolog
UniProt Protein Name:	Vacuolar protein sorting-associated protein 16 homolog
Protein Family:	Vacuolar protein sorting-associated protein
UniProt Gene Name:	VPS16

Western blot analysis of extracts of various cell lines, using VPS16 antibody (CAB15888) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)