Anti-VMP1 Antibody (CAB15523)
- SKU:
- CAB15523
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Autophagy
Frequently bought together:
Description
| Antibody Name: | Anti-VMP1 Antibody |
| Antibody SKU: | CAB15523 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human VMP1 (NP_112200.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:200 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse kidney |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human VMP1 (NP_112200.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | KMHI QKIF VIIT FSKH IVEQ MVAF IGAV PGIG PSLQ KPFQ EYLE AQRQ KLHH KSEM GTPQ GENW LSWM FEKL VVVM VCYF ILSI INSM AQSY AKRI QQRL NSEE KTK |
| Gene ID: | 81671 |
| Uniprot: | Q96GC9 |
| Cellular Location: | Cell membrane, Endoplasmic reticulum, Endoplasmic reticulum-Golgi intermediate compartment membrane, Multi-pass membrane protein, Vacuole membrane |
| Calculated MW: | 24kDa/46kDa |
| Observed MW: | 42kDa |
| Synonyms: | VMP1, EPG3, TANGO5, TMEM49 |
| Background: |
| UniProt Protein Function: | VMP1: Stress-induced protein that, when overexpressed, promotes formation of intracellular vacuoles followed by cell death. May be involved in the cytoplasmic vacuolization of acinar cells during the early stage of acute pancreatitis. Plays a role in the initial stages of the autophagic process through its interaction with BECN1. Involved in cell-cell adhesion. Plays an essential role in formation of cell junctions. Interacts with BECN1. Interacts with TJP1. Interacts with TP53INP2. Belongs to the VMP1 family. |
| UniProt Protein Details: | Protein type:Endoplasmic reticulum; Membrane protein, integral; Membrane protein, multi-pass Chromosomal Location of Human Ortholog: 17q23.1 Cellular Component: endoplasmic reticulum; membrane; nucleolus Molecular Function:protein binding Biological Process: cell-cell adhesion; endoplasmic reticulum organization and biogenesis; exocytosis; Golgi organization and biogenesis; regulation of autophagy |
| NCBI Summary: | This gene encodes a transmembrane protein that plays a key regulatory role in the process of autophagy. The ectopic overexpression of the encoded protein in cultured cells triggers autophagy even under nutrient-rich conditions. This gene is overexpressed in pancreatitis affected acinar cells where the encoded protein mediates sequestration and degradation of potentially deleterious activated zymogen granules in a process termed, zymophagy. [provided by RefSeq, Jul 2016] |
| UniProt Code: | Q96GC9 |
| NCBI GenInfo Identifier: | 74731809 |
| NCBI Gene ID: | 81671 |
| NCBI Accession: | Q96GC9.1 |
| UniProt Secondary Accession: | Q96GC9,Q9H0P4, Q9P089, B4DVV9, |
| UniProt Related Accession: | Q96GC9 |
| Molecular Weight: | 24,148 Da |
| NCBI Full Name: | Vacuole membrane protein 1 |
| NCBI Synonym Full Names: | vacuole membrane protein 1 |
| NCBI Official Symbol: | VMP1 |
| NCBI Official Synonym Symbols: | EPG3; TANGO5; TMEM49 |
| NCBI Protein Information: | vacuole membrane protein 1 |
| UniProt Protein Name: | Vacuole membrane protein 1 |
| UniProt Synonym Protein Names: | Transmembrane protein 49 |
| Protein Family: | Vacuole membrane protein |
| UniProt Gene Name: | VMP1 |
| UniProt Entry Name: | VMP1_HUMAN |
 | Western blot analysis of extracts of mouse kidney, using VMP1 antibody (CAB15523) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
 | Immunofluorescence analysis of NIH-3T3 cells using VMP1 Polyclonal Antibody (CAB15523) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of U-2 OS cells using VMP1 Polyclonal Antibody (CAB15523) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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