Anti-VISTA Antibody (CAB10419)
- SKU:
- CAB10419
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Frequently bought together:
Description
| Antibody Name: | Anti-VISTA Antibody |
| Antibody SKU: | CAB10419 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 33-194 of human VISTA (NP_071436.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | HeLa, LO2, A-549 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 33-194 of human VISTA (NP_071436.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | FKVA TPYS LYVC PEGQ NVTL TCRL LGPV DKGH DVTF YKTW YRSS RGEV QTCS ERRP IRNL TFQD LHLH HGGH QAAN TSHD LAQR HGLE SASD HHGN FSIT MRNL TLLD SGLY CCLV VEIR HHHS EHRV HGAM ELQV QTGK DAPS NCVV YPSS SQDS ENIT AA |
| Gene ID: | 64115 |
| Uniprot: | Q9H7M9 |
| Cellular Location: | Cell membrane, Single-pass type I membrane protein |
| Calculated MW: | 33kDa |
| Observed MW: | 37kDa |
| Synonyms: | VSIR, B7-H5, B7H5, C10orf54, DD1alpha, GI24, PD-1H, PP2135, SISP1, VISTA |
| Background: |
| UniProt Protein Function: | VISTA: May stimulate MMP14-mediated MMP2 activation |
| UniProt Protein Details: | Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 10q22.1 Cellular Component: cell surface; intercellular junction; plasma membrane Biological Process: cell-cell adhesion |
| UniProt Code: | Q9H7M9 |
| NCBI GenInfo Identifier: | 62339432 |
| NCBI Gene ID: | 64115 |
| NCBI Accession: | NP_071436.1 |
| UniProt Related Accession: | Q9H7M9 |
| Molecular Weight: | 34kDa |
| NCBI Full Name: | V-type immunoglobulin domain-containing suppressor of T-cell activation |
| NCBI Synonym Full Names: | V-set immunoregulatory receptor |
| NCBI Official Symbol: | VSIR |
| NCBI Official Synonym Symbols: | B7H5; GI24; B7-H5; Dies1; PD-1H; SISP1; VISTA; PP2135; C10orf54; DD1alpha |
| NCBI Protein Information: | V-type immunoglobulin domain-containing suppressor of T-cell activation |
| UniProt Protein Name: | V-type immunoglobulin domain-containing suppressor of T-cell activation |
| UniProt Synonym Protein Names: | Platelet receptor Gi24 |
| UniProt Gene Name: | C10orf54 |
| UniProt Entry Name: | VISTA_HUMAN |
 | Western blot analysis of extracts of various cell lines, using VISTA antibody (CAB10419) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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