Anti-VIMP 항체 (CAB15013)

Antibody Name:	Anti-VIMP Antibody
Antibody SKU:	CAB15013
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 50-150 of human VIMP (NP_982298.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, A-431, Mouse brain, Rat lung

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 50-150 of human VIMP (NP_982298.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	KLSA RLRA LRQR QLDR AAAA VEPD VVVK RQEA LAAA RLKM QEEL NAQV EKHK EKLK QLEE EKRR QKIE MWDS MQEG KSYK GNAK KPQE EDSP GPST SSVL K
Gene ID:	55829
Uniprot:	Q9BQE4
Cellular Location:	Cytoplasm, Endoplasmic reticulum membrane, Single-pass membrane protein
Calculated MW:	21kDa
Observed MW:	21kDa

Synonyms:

SELENOS, AD-015, ADO15, SBBI8, SELS, SEPS1, VIMP

Background:

This gene encodes a transmembrane protein that is localized in the endoplasmic reticulum (ER). It is involved in the degradation process of misfolded proteins in the ER, and may also have a role in inflammation control. This protein is a selenoprotein, containing the rare amino acid selenocysteine (Sec). Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Two additional phylogenetically conserved stem-loop structures (Stem-loop 1 and Stem-loop 2) in the 3' UTR have been shown to function as modulators of Sec insertion in this protein (PMID:23614019). An alternatively spliced transcript variant, lacking the SECIS element and encoding a non-Sec containing shorter isoform, has been described for this gene.

UniProt Protein Function:	Involved in the degradation process of misfolded endoplasmic reticulum (ER) luminal proteins. Participates in the transfer of misfolded proteins from the ER to the cytosol, where they are destroyed by the proteasome in a ubiquitin-dependent manner. Probably acts by serving as a linker between DERL1, which mediates the retrotranslocation of misfolded proteins into the cytosol, and the ATPase complex VCP, which mediates the translocation and ubiquitination.
NCBI Summary:	This gene encodes a transmembrane protein that is localized in the endoplasmic reticulum (ER). It is involved in the degradation process of misfolded proteins in the ER, and may also have a role in inflammation control. This protein is a selenoprotein, containing the rare amino acid selenocysteine (Sec). Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Two additional phylogenetically conserved stem-loop structures (Stem-loop 1 and Stem-loop 2) in the 3' UTR of this mRNA have been shown to function as modulators of Sec insertion. An alternatively spliced transcript variant, lacking the SECIS element and encoding a non-Sec containing shorter isoform, has been described for this gene (PMID:23614019). [provided by RefSeq, Jul 2017]
UniProt Code:	Q9BQE4
NCBI GenInfo Identifier:	33285002
NCBI Gene ID:	55829
NCBI Accession:	NP_060915.2
UniProt Secondary Accession:	Q9BQE4,Q3B771, Q9P0I6,
UniProt Related Accession:	Q9BQE4
Molecular Weight:	Calculated: 21kDaObserved: 21kDa
NCBI Full Name:	selenoprotein S isoform 1
NCBI Synonym Full Names:	selenoprotein S
NCBI Official Symbol:	SELENOS
NCBI Official Synonym Symbols:	SELS; VIMP; ADO15; SBBI8; SEPS1; AD-015
NCBI Protein Information:	selenoprotein S
UniProt Protein Name:	Selenoprotein S
UniProt Synonym Protein Names:	VCP-interacting membrane protein
Protein Family:	Selenoprotein
UniProt Gene Name:	SELENOS

Western blot analysis of extracts of various cell lines, using VIMP antibody (CAB15013) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90S.

ELISA Kits

Human Selenoprotein S (SELS) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)