Anti-VILL Antibody (CAB15440)
- SKU:
- CAB15440
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-VILL Antibody |
| Antibody SKU: | CAB15440 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 637-856 of human VILL (NP_056957.3). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:200 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse kidney, Mouse eye, Mouse lung, Rat kidney, Rat eye |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 637-856 of human VILL (NP_056957.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | LDKY DIML LDTW QEIF LWLG EAAS EWKE AVAW GQEY LKTH PAGR SPAT PIVL VKQG HEPP TFIG WFFT WDPY KWTS HPSH KEVV DGSP AAAS TISE ITAE VNNL RLSR WPGN GRAG AVAL QALK GSQD SSEN DLVR SPKS AGSR TSSS VSST SATI NGGL RREQ LMHQ AVED LPEG VDPA RREF YLSD SDFQ DIFG KSKE EFYS MATW RQRQ EKKQ LGFF |
| Gene ID: | 50853 |
| Uniprot: | O15195 |
| Cellular Location: | |
| Calculated MW: | 94kDa/95kDa |
| Observed MW: | 96kDa |
| Synonyms: | VILL |
| Background: | The protein encoded by this gene belongs to the villin/gelsolin family. It contains 6 gelsolin-like repeats and a headpiece domain. It may play a role in actin-bundling. |
| UniProt Protein Function: | VILL: Possible tumor suppressor. Belongs to the villin/gelsolin family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 3p21.3 Cellular Component: actin cytoskeleton Molecular Function:structural constituent of cytoskeleton |
| NCBI Summary: | The protein encoded by this gene belongs to the villin/gelsolin family. It contains 6 gelsolin-like repeats and a headpiece domain. It may play a role in actin-bundling. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O15195 |
| NCBI GenInfo Identifier: | 61252134 |
| NCBI Gene ID: | 50853 |
| NCBI Accession: | O15195.3 |
| UniProt Secondary Accession: | O15195,Q9BT80, Q9BWH7, A8MZP1, |
| UniProt Related Accession: | O15195 |
| Molecular Weight: | 94,417 Da |
| NCBI Full Name: | Villin-like protein |
| NCBI Synonym Full Names: | villin like |
| NCBI Official Symbol: | VILL |
| NCBI Protein Information: | villin-like protein |
| UniProt Protein Name: | Villin-like protein |
| Protein Family: | Villin |
| UniProt Gene Name: | VILL |
| UniProt Entry Name: | VILL_HUMAN |
 | Western blot analysis of extracts of various cell lines, using VILL Rabbit pAb (CAB15440) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of L929 cells using VILL antibody (CAB15440) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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