Anti-VEGF Receptor 2 항체 (CAB11127)

Antibody Name:	Anti-VEGF Receptor 2 Antibody
Antibody SKU:	CAB11127
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 20-200 of human VEGF Receptor 2 (NP_002244.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	mouse heart, mouse liver, mouse lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 20-200 of human VEGF Receptor 2 (NP_002244.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	ASVG LPSV SLDL PRLS IQKD ILTI KANT TLQI TCRG QRDL DWLW PNNQ SGSE QRVE VTEC SDGL FCKT LTIP KVIG NDTG AYKC FYRE TDLA SVIY VYVQ DYRS PFIA SVSD QHGV VYIT ENKN KTVV IPCL GSIS NLNV SLCA RYPE KRFV PDGN RISW DSKK GFTI PSYM ISYA GMVF C
Gene ID:	3791
Uniprot:	P35968
Cellular Location:	Cell junction, Cell membrane, Cytoplasm, Cytoplasmic vesicle, Early endosome, Endoplasmic reticulum, Nucleus, Secreted, Single-pass type I membrane protein
Calculated MW:	75kDa/79kDa/151kDa
Observed MW:	240kDa

Synonyms:

CD309, FLK1, VEGFR, VEGFR2, VEGF Receptor 2, KDR

Background:

Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc.. Mutations of this gene are implicated in infantile capillary hemangiomas.

UniProt Protein Function:	VEGFR2: a receptor tyrosine kinase of the VEGFR family. High affinity receptor for VEGF and VEGF-C. Ligand binding induces autophosphorylation and activation. Activated receptor recruits proteins including Shc, GRB2, PI3K, Nck, SHP-1 and SHP-2. Plays a key role in vascular development and regulation of vascular permeability.
UniProt Protein Details:	Protein type:Protein kinase, TK; Kinase, protein; Membrane protein, integral; EC 2.7.10.1; Protein kinase, tyrosine (receptor); TK group; VEGFR family Chromosomal Location of Human Ortholog: 4q11-q12 Cellular Component: Golgi apparatus; endoplasmic reticulum; integral to plasma membrane; early endosome; cytoplasmic membrane-bound vesicle; extracellular region; plasma membrane; cell junction; nucleus; endosome; lipid raft; external side of plasma membrane Molecular Function:integrin binding; vascular endothelial growth factor receptor activity; protein binding; growth factor binding; protein-tyrosine kinase activity; receptor signaling protein tyrosine kinase activity; Hsp90 protein binding; transmembrane receptor protein tyrosine kinase activity; ATP binding Biological Process: extracellular matrix organization and biogenesis; positive regulation of positive chemotaxis; peptidyl-tyrosine phosphorylation; viral reproduction; protein amino acid autophosphorylation; cell maturation; calcium ion homeostasis; regulation of cell shape; positive regulation of MAPKKK cascade; positive regulation of focal adhesion formation; ovarian follicle development; positive regulation of cell proliferation; positive regulation of mesenchymal cell proliferation; angiogenesis; vasculogenesis; endothelial cell differentiation; cell fate commitment; embryonic hemopoiesis; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of angiogenesis; cell migration during sprouting angiogenesis; branching morphogenesis of a tube; positive regulation of endothelial cell proliferation; positive regulation of protein amino acid phosphorylation; lymph vessel development; vascular endothelial growth factor receptor signaling pathway; alveolus development; surfactant homeostasis; transmembrane receptor protein tyrosine kinase signaling pathway; negative regulation of apoptosis; positive regulation of cell migration; positive regulation of nitric-oxide synthase biosynthetic process Disease: Hemangioma, Capillary Infantile
NCBI Summary:	Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc.. Mutations of this gene are implicated in infantile capillary hemangiomas. [provided by RefSeq, May 2009]
UniProt Code:	P35968
NCBI GenInfo Identifier:	9087218
NCBI Gene ID:	3791
NCBI Accession:	P35968.2
UniProt Secondary Accession:	P35968,O60723, Q14178, A2RRS0, B5A925, C5IFA0,
UniProt Related Accession:	P35968
Molecular Weight:	79,634 Da
NCBI Full Name:	Vascular endothelial growth factor receptor 2
NCBI Synonym Full Names:	kinase insert domain receptor (a type III receptor tyrosine kinase)
NCBI Official Symbol:	KDR
NCBI Official Synonym Symbols:	FLK1; CD309; VEGFR; VEGFR2
NCBI Protein Information:	vascular endothelial growth factor receptor 2; soluble VEGFR2; fetal liver kinase 1; fetal liver kinase-1; protein-tyrosine kinase receptor Flk-1; tyrosine kinase growth factor receptor
UniProt Protein Name:	Vascular endothelial growth factor receptor 2
UniProt Synonym Protein Names:	Fetal liver kinase 1; FLK-1; Kinase insert domain receptor; KDR; Protein-tyrosine kinase receptor flk-1; CD_antigen: CD309
Protein Family:	Vascular endothelial growth factor receptor
UniProt Gene Name:	KDR
UniProt Entry Name:	VGFR2_HUMAN

	Western blot analysis of extracts of various cell lines, using VEGF Receptor 2 antibody (CAB11127) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded rat testis using VEGF Receptor 2 antibody (CAB11127) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse testis using VEGF Receptor 2 antibody (CAB11127) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse brain using VEGF Receptor 2 antibody (CAB11127) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
Anti-VEGF Receptor 2 Antibody (CAB5609)	Human VEGFR-2/KDR (Vascular Endothelial Growth Factor Receptor 2) CLIA Kit (HUES00913)
Anti-VEGF Receptor 2 Antibody (CAB7695)	Human Vascular endothelial growth factor receptor 2 (KDR) ELISA Kit
Anti-Phospho-KDR-Y1175 Antibody (CABP0382)	VEGFR2 (Phospho-Tyr951) Colorimetric Cell-Based ELISA Kit
Anti-Phospho-VEGFR2-Y996 Antibody (CABP0595)	VEGFR2 Colorimetric Cell-Based ELISA Kit
Anti-Phospho-VEGF Receptor 2-Y1175 Antibody (CABP1095)	VEGFR2 (Phospho-Tyr1175) Colorimetric Cell-Based ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)