Anti-VCPIP1 Antibody (CAB16572)
- SKU:
- CAB16572
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Frequently bought together:
Description
| Antibody Name: | Anti-VCPIP1 Antibody |
| Antibody SKU: | CAB16572 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 923-1222 of human VCPIP1 (NP_079330.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | MCF7, HeLa |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 923-1222 of human VCPIP1 (NP_079330.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | VFYS IMKK TMGM ADGK HCTF PHLP GKTF VYNA SEDR LELC VDAA GHFP IGPD VEDL VKEA VSQV RAEA TTRS RESS PSHG LLKL GSGG VVKK KSEQ LHNV TAFQ GKGH SLGT ASGN PHLD PRAR ETSV VRKH NTGT DFSN SSTK TEPS VFTA SSSN SELI RIAP GVVT MRDG RQLD PDLV EAQR KKLQ EMVS SIQA SMDR HLRD QSTE QSPS DLPQ RKTE VVSS SAKS GSLQ TGLP ESFP LTGG TENL NTET TDGC VADA LGAA FATR SKAQ RGNS VEEL EEMD SQDA EMTN TTEP MDHS |
| Gene ID: | 80124 |
| Uniprot: | Q96JH7 |
| Cellular Location: | Endoplasmic reticulum, Golgi apparatus, Golgi stack |
| Calculated MW: | 134kDa |
| Observed MW: | 134kDa |
| Synonyms: | VCPIP1, DUBA3, VCIP135 |
| Background: |
| UniProt Protein Function: | VCIP135: Acts as a deubiquitinating enzyme. Necessary for VCP- mediated reassembly of Golgi stacks after mitosis. May play a role in VCP-mediated formation of transitional endoplasmic reticulum (tER). Mediates dissociation of the ternary complex containing STX5A, NSFL1C and VCP. |
| UniProt Protein Details: | Protein type:Ubiquitin conjugating system; Protease; EC 3.4.19.12; Cell cycle regulation; Ubiquitin-specific protease Chromosomal Location of Human Ortholog: 8q13 Cellular Component: cytoplasm Molecular Function:ubiquitin-specific protease activity Biological Process: protein ubiquitination |
| UniProt Code: | Q96JH7 |
| NCBI GenInfo Identifier: | 42560002 |
| NCBI Gene ID: | 80124 |
| NCBI Accession: | Q96JH7.2 |
| UniProt Secondary Accession: | Q96JH7,Q504T4, Q86T93, Q86W01, Q8N3A9, Q9H5R8, |
| UniProt Related Accession: | Q96JH7 |
| Molecular Weight: | 134,321 Da |
| NCBI Full Name: | Deubiquitinating protein VCIP135 |
| NCBI Synonym Full Names: | valosin containing protein interacting protein 1 |
| NCBI Official Symbol: | VCPIP1 |
| NCBI Official Synonym Symbols: | DUBA3; VCIP135 |
| NCBI Protein Information: | deubiquitinating protein VCIP135 |
| UniProt Protein Name: | Deubiquitinating protein VCIP135 |
| UniProt Synonym Protein Names: | Valosin-containing protein p97/p47 complex-interacting protein 1; Valosin-containing protein p97/p47 complex-interacting protein p135; VCP/p47 complex-interacting 135-kDa protein |
| Protein Family: | Deubiquitinating protein |
| UniProt Gene Name: | VCPIP1 |
| UniProt Entry Name: | VCIP1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using VCPIP1 antibody (CAB16572) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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