Anti-VCP Antibody (CAB13368)
- SKU:
- CAB13368
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Autophagy
Description
| Antibody Name: | Anti-VCP Antibody |
| Antibody SKU: | CAB13368 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 647-806 of human VCP (NP_009057.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, K-562, SH-SY5Y, Mouse brain, Mouse spleen, Rat liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 647-806 of human VCP (NP_009057.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | LPDE KSRV AILK ANLR KSPV AKDV DLEF LAKM TNGF SGAD LTEI CQRA CKLA IRES IESE IRRE RERQ TNPS AMEV EEDD PVPE IRRD HFEE AMRF ARRS VSDN DIRK YEMF AQTL QQSR GFGS FRFP SGNQ GGAG PSQG SGGG TGGS VYTE DNDD DLYG |
| Gene ID: | 7415 |
| Uniprot: | P55072 |
| Cellular Location: | Cytoplasm, Endoplasmic reticulum, Nucleus, cytosol |
| Calculated MW: | 89kDa |
| Observed MW: | 90KDa |
| Synonyms: | VCP, ALS14, CDC48, CMT2Y, HEL-220, HEL-S-70, IBMPFD, IBMPFD1, TERA, p97 |
| Background: | The protein encoded by this gene is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. This protein, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation. |
| UniProt Protein Function: | VCP: valosin-containing protein (VCP) is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. VCP, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. Necessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis. Involved in the formation of the nuclear envelope and of the transitional endoplasmic reticulum (tER). Regulates NFKappaB pathway, which is important for metastasis of osteosarcoma. Tyrosine phosphorylation regulates its cell cycle-dependent nuclear localization. |
| UniProt Protein Details: | Protein type:Endoplasmic reticulum; EC 3.6.4.6; DNA repair, damage; Chaperone; Hydrolase Chromosomal Location of Human Ortholog: 9p13.3 Cellular Component: nucleoplasm; proteasome complex; endoplasmic reticulum membrane; intracellular membrane-bound organelle; perinuclear region of cytoplasm; endoplasmic reticulum; cytoplasm; lipid particle; cytosol; nucleus Molecular Function:protein domain specific binding; identical protein binding; protein binding; ATPase activity; protein complex binding; ADP binding; polyubiquitin binding; protein phosphatase binding; lipid binding; ATP binding; receptor binding Biological Process: caspase activation; proteasomal ubiquitin-dependent protein catabolic process; ER to Golgi vesicle-mediated transport; ER-associated protein catabolic process; unfolded protein response; protein ubiquitination; DNA repair; regulation of apoptosis; retrograde protein transport, ER to cytosol; bypass DNA synthesis; establishment of protein localization; positive regulation of protein complex assembly; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; double-strand break repair; positive regulation of protein catabolic process; protein amino acid N-linked glycosylation via asparagine; response to DNA damage stimulus; protein homooligomerization Disease: Amyotrophic Lateral Sclerosis 14, With Or Without Frontotemporal Dementia; Inclusion Body Myopathy With Early-onset Paget Disease With Or Without Frontotemporal Dementia 1 |
| NCBI Summary: | The protein encoded by this gene is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. This protein, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P55072 |
| NCBI GenInfo Identifier: | 6094447 |
| NCBI Gene ID: | 7415 |
| NCBI Accession: | P55072.4 |
| UniProt Secondary Accession: | P55072,Q0V924, Q2TAI5, Q969G7, Q9UCD5, B2R5T8, |
| UniProt Related Accession: | P55072 |
| Molecular Weight: | 89,322 Da |
| NCBI Full Name: | Transitional endoplasmic reticulum ATPase |
| NCBI Synonym Full Names: | valosin containing protein |
| NCBI Official Symbol: | VCP |
| NCBI Official Synonym Symbols: | p97; TERA; ALS14; IBMPFD |
| NCBI Protein Information: | transitional endoplasmic reticulum ATPase; TER ATPase; yeast Cdc48p homolog; valosin-containing protein; 15S Mg(2+)-ATPase p97 subunit |
| UniProt Protein Name: | Transitional endoplasmic reticulum ATPase |
| UniProt Synonym Protein Names: | 15S Mg(2+)-ATPase p97 subunit; Valosin-containing protein |
| Protein Family: | Transitional endoplasmic reticulum ATPase |
| UniProt Gene Name: | VCP |
| UniProt Entry Name: | TERA_HUMAN |
 | Western blot analysis of extracts of various cell lines, using VCP antibody (CAB13368) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded human placenta using VCP Rabbit pAb (CAB13368) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse spinal cord using VCP Rabbit pAb (CAB13368) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat kidney using VCP Rabbit pAb (CAB13368) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of C6 cells using VCP antibody (CAB13368) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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