Anti-USP47 Antibody (CAB15461)

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SKU:
CAB15461
Product type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
Frequently bought together:

Description

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Antibody Name:Anti-USP47 Antibody
Antibody SKU:CAB15461
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1128-1287 of human USP47 (NP_060414.3).
Application:WB IHC
Recommended Dilution:WB 1:200 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse
Positive Samples:Jurkat, HeLa, SGC-7901, Mouse brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1128-1287 of human USP47 (NP_060414.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:VEKM KSMS QLAV LSRR WKPS EMKL DPFQ EVVL ESSS VDEL REKL SEIS GIPL DDIE FAKG RGTF PCDI SVLD IHQD LDWN PKVS TLNV WPLY ICDD GAVI FYRD KTEE LMEL TDEQ RNEL MKKE SSRL QKTG HRVT YSPR KEKA LKIY LDGA PNKD LTQD
Gene ID:55031
Uniprot:Q96K76
Cellular Location:Cytoplasm
Calculated MW:18kDa/147kDa/154kDa/157kDa
Observed MW:180kDa
Synonyms:USP47, TRFP
Background:
UniProt Protein Function:USP47: Ubiquitin-specific protease that specifically deubiquitinates monoubiquitinated DNA polymerase beta (POLB), stabilizing POLB thereby playing a role in base-excision repair (BER). Acts as a regulator of cell growth and genome integrity. May also indirectly regulates CDC25A expression at a transcriptional level. Belongs to the peptidase C19 family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 3.4.19.12; Ubiquitin-specific protease; Motility/polarity/chemotaxis; Actin-binding; Protease; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 11p15.3

Cellular Component: cytoplasm; nucleus; SCF ubiquitin ligase complex

Molecular Function:protein binding; ubiquitin-specific protease activity

Biological Process: base-excision repair; negative regulation of apoptosis; negative regulation of caspase activity; negative regulation of transcription, DNA-dependent; positive regulation of cell growth; response to DNA damage stimulus; response to drug

UniProt Code:Q96K76
NCBI GenInfo Identifier:313104266
NCBI Gene ID:55031
NCBI Accession:Q96K76.3
UniProt Secondary Accession:Q96K76,Q658U0, Q86Y73, Q8TEP6, Q9BWI0, Q9NWN1, B3KXF5 E9PM46,
UniProt Related Accession:Q96K76
Molecular Weight:154,697 Da
NCBI Full Name:Ubiquitin carboxyl-terminal hydrolase 47
NCBI Synonym Full Names:ubiquitin specific peptidase 47
NCBI Official Symbol:USP47  
NCBI Official Synonym Symbols:TRFP  
NCBI Protein Information:ubiquitin carboxyl-terminal hydrolase 47
UniProt Protein Name:Ubiquitin carboxyl-terminal hydrolase 47
UniProt Synonym Protein Names:Deubiquitinating enzyme 47; Ubiquitin thioesterase 47; Ubiquitin-specific-processing protease 47
Protein Family:Ubiquitin carboxyl-terminal hydrolase
UniProt Gene Name:USP47  
UniProt Entry Name:UBP47_HUMAN
Anti-USP47 Antibody (CAB15461)Western blot analysis of extracts of various cell lines, using USP47 Rabbit pAb (CAB15461) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
Anti-USP47 Antibody (CAB15461)Immunohistochemistry of paraffin-embedded Human colon carcinoma using USP47 Rabbit pAb (CAB15461) at dilution of 1:100 (40x lens).

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