Anti-USP21 Antibody (CAB16663)
- SKU:
- CAB16663
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Frequently bought together:
Description
| Antibody Name: | Anti-USP21 Antibody |
| Antibody SKU: | CAB16663 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 400-500 of human USP21 (NP_001014443.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse |
| Positive Samples: | LO2, 293T |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 400-500 of human USP21 (NP_001014443.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | LSLP IPKK GFAG GKVS LRDC FNLF TKEE ELES ENAP VCDR CRQK TRST KKLT VQRF PRIL VLHL NRFS ASRG SIKK SSVG VDFP LQRL SLGD FASD KAGS P |
| Gene ID: | 27005 |
| Uniprot: | Q9UK80 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 42kDa/61kDa/62kDa |
| Observed MW: | 63kDa |
| Synonyms: | USP21, USP16, USP23 |
| Background: | This gene encodes a member of the C19 peptidase family, also known as family 2 of ubiquitin carboxy-terminal hydrolases. The encoded protein cleaves ubiquitin from ubiquitinated proteins for recycling in intracellular protein degradation. The encoded protein is also able to release NEDD8, a ubiquitin-like protein, from NEDD8-conjugated proteins. This gene has been referred to as USP16 and USP23 but is now known as USP21. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | USP21: Deubiquitinates histone H2A, a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A releaves the repression of di- and trimethylation of histone H3 at 'Lys-4', resulting in regulation of transcriptional initiation. Regulates gene expression via histone H2A deubiquitination. Also capable of removing NEDD8 from NEDD8 conjugates but has no effect on Sentrin-1 conjugates. Belongs to the peptidase C19 family. USP21 subfamily. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Protease; Ubiquitin-specific protease; EC 3.4.19.12; Ubiquitin conjugating system Chromosomal Location of Human Ortholog: 1q22 Cellular Component: cytoplasm; nucleoplasm; plasma membrane Molecular Function:cysteine-type peptidase activity; NEDD8-specific protease activity; protein binding; transcription coactivator activity; ubiquitin-specific protease activity Biological Process: histone deubiquitination; positive regulation of transcription, DNA-dependent |
| NCBI Summary: | This gene encodes a member of the C19 peptidase family, also known as family 2 of ubiquitin carboxy-terminal hydrolases. The encoded protein cleaves ubiquitin from ubiquitinated proteins for recycling in intracellular protein degradation. The encoded protein is also able to release NEDD8, a ubiquitin-like protein, from NEDD8-conjugated proteins. This gene has been referred to as USP16 and USP23 but is now known as USP21. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2016] |
| UniProt Code: | Q9UK80 |
| NCBI GenInfo Identifier: | 10720334 |
| NCBI Gene ID: | 27005 |
| NCBI Accession: | Q9UK80.1 |
| UniProt Secondary Accession: | Q9UK80,Q59H60, Q5BKT5, Q5VTW9, Q5VTX0, Q9BTV1, Q9HBS2 Q9NYN4, |
| UniProt Related Accession: | Q9UK80 |
| Molecular Weight: | 61,122 Da |
| NCBI Full Name: | Ubiquitin carboxyl-terminal hydrolase 21 |
| NCBI Synonym Full Names: | ubiquitin specific peptidase 21 |
| NCBI Official Symbol: | USP21 |
| NCBI Official Synonym Symbols: | USP16; USP23 |
| NCBI Protein Information: | ubiquitin carboxyl-terminal hydrolase 21 |
| UniProt Protein Name: | Ubiquitin carboxyl-terminal hydrolase 21 |
| UniProt Synonym Protein Names: | Deubiquitinating enzyme 21; Ubiquitin thioesterase 21; Ubiquitin-specific-processing protease 21 |
| UniProt Gene Name: | USP21 |
| UniProt Entry Name: | UBP21_HUMAN |
 | Western blot analysis of extracts of various cell lines, using USP21 antibody (CAB16663) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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