Anti-USP13 Antibody (CAB10264)
- SKU:
- CAB10264
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Autophagy
Frequently bought together:
Description
| Antibody Name: | Anti-USP13 Antibody |
| Antibody SKU: | CAB10264 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 90-200 of human USP13 (NP_003931.2). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:1000 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse heart, Mouse brain, Rat heart, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 90-200 of human USP13 (NP_003931.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MHLK RHVR EKVR GASG GALP KRRN SKIF LDLD TDDD LNSD DYEY EDEA KLVI FPDH YEIA LPNI EELP ALVT IACD AVLS SKSP YRKQ DPDT WENE LPVS KYAN NLTQ LDN |
| Gene ID: | 8975 |
| Uniprot: | Q92995 |
| Cellular Location: | |
| Calculated MW: | 90kDa/97kDa |
| Observed MW: | 97kDa |
| Synonyms: | USP13, ISOT3, IsoT-3 |
| Background: |
| UniProt Protein Function: | USP13: Deubiquitinase that mediates deubiquitination of target proteins such as BECN1, MITF, SKP2 and USP10 and is involved in various processes such as autophagy and endoplasmic reticulum- associated degradation (ERAD). Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. Also deubiquitinates USP10, an essential regulator of p53/TP53 stability. In turn, PIK3C3/VPS34- containing complexes regulate USP13 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. Recruited by nuclear UFD1 and mediates deubiquitination of SKP2, thereby regulating endoplasmic reticulum-associated degradation (ERAD). Mediates stabilization of SIAH2 independently of deubiquitinase activity: binds ubiquitinated SIAH2 and acts by impairing SIAH2 autoubiquitination. Has a weak deubiquitinase activity in vitro and preferentially cleaves 'Lys-63'-linked polyubiquitin chains. In contrast to USP5, it is not able to mediate unanchored polyubiquitin disassembly. Able to cleave ISG15 in vitro; however, additional experiments are required to confirm such data. Belongs to the peptidase C19 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 3.4.19.12; Protease; Ubiquitin conjugating system; Ubiquitin-specific protease Chromosomal Location of Human Ortholog: 3q26.33 Cellular Component: cytosol; nucleoplasm Molecular Function:chaperone binding; cysteine-type endopeptidase activity; protein binding; ubiquitin binding; ubiquitin protein ligase binding; ubiquitin-specific protease activity Biological Process: cell proliferation; melanocyte differentiation; protein deubiquitination; protein stabilization; regulation of autophagy; regulation of transcription, DNA-dependent |
| UniProt Code: | Q92995 |
| NCBI GenInfo Identifier: | 209572692 |
| NCBI Gene ID: | 8975 |
| NCBI Accession: | Q92995.2 |
| UniProt Secondary Accession: | Q92995,Q96B25, A8K2S3, B4DYF3, D3DNS2, |
| UniProt Related Accession: | Q92995 |
| Molecular Weight: | 97kDa |
| NCBI Full Name: | Ubiquitin carboxyl-terminal hydrolase 13 |
| NCBI Synonym Full Names: | ubiquitin specific peptidase 13 (isopeptidase T-3) |
| NCBI Official Symbol: | USP13 |
| NCBI Official Synonym Symbols: | ISOT3; IsoT-3 |
| NCBI Protein Information: | ubiquitin carboxyl-terminal hydrolase 13 |
| UniProt Protein Name: | Ubiquitin carboxyl-terminal hydrolase 13 |
| UniProt Synonym Protein Names: | Deubiquitinating enzyme 13; Isopeptidase T-3; ISOT-3; Ubiquitin thioesterase 13; Ubiquitin-specific-processing protease 13 |
| UniProt Gene Name: | USP13 |
 | Western blot analysis of extracts of various cell lines, using USP13 antibody (CAB10264) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
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