Anti-USP1 항체 (CAB6785)

Antibody Name:	Anti-USP1 Antibody
Antibody SKU:	CAB6785
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 486-785 of human USP1 (NP_003359.3).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse
Positive Samples:	293T, HepG2, MCF7, Mouse testis, Mouse spleen, Mouse brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 486-785 of human USP1 (NP_003359.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	RWAI SQFA SVER IVGE DKYF CENC HHYT EAER SLLF DKMP EVIT IHLK CFAA SGLE FDCY GGGL SKIN TPLL TPLK LSLE EWST KPTN DSYG LFAV VMHS GITI SSGH YTAS VKVT DLNS LELD KGNF VVDQ MCEI GKPE PLNE EEAR GVVE NYND EEVS IRVG GNTQ PSKV LNKK NVEA IGLL GGQK SKAD YELY NKAS NPDK VAST AFAE NRNS ETSD TTGT HESD RNKE SSDQ TGIN ISGF ENKI SYVV QSLK EYEG KWLL FDDS EVKV TEEK DFLN SLSP STSP TSTP YLLF YKKL
Gene ID:	7398
Uniprot:	O94782
Cellular Location:	Nucleus
Calculated MW:	88kDa
Observed MW:	115kDa

Synonyms:

USP1, UBP

Background:

This gene encodes a member of the ubiquitin-specific processing (UBP) family of proteases that is a deubiquitinating enzyme (DUB) with His and Cys domains. This protein is located in the cytoplasm and cleaves the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins. The protein specifically deubiquitinates a protein in the Fanconi anemia (FA) DNA repair pathway. Alternate transcriptional splice variants have been characterized.

UniProt Protein Function:	USP1: Negative regulator of DNA damage repair which specifically deubiquitinates monoubiquitinated FANCD2. Also involved in PCNA-mediated translesion synthesis (TLS) by deubiquitinating monoubiquitinated PCNA. Has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have a high activity. Interacts with FANCD2 and PCNA. Interacts with WDR48. Down-regulated following DNA damage. Belongs to the peptidase C19 family.
UniProt Protein Details:	Protein type:EC 3.4.19.12; Ubiquitin conjugating system; Ubiquitin-specific protease; Protease Chromosomal Location of Human Ortholog: 1p31.3 Cellular Component: nucleoplasm; nucleus Molecular Function:protein binding; cysteine-type endopeptidase activity; ubiquitin-specific protease activity Biological Process: regulation of DNA repair; proteasomal ubiquitin-dependent protein catabolic process; protein deubiquitination; DNA repair; skeletal development; response to UV
NCBI Summary:	This gene encodes a member of the ubiquitin-specific processing (UBP) family of proteases that is a deubiquitinating enzyme (DUB) with His and Cys domains. This protein is located in the cytoplasm and cleaves the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins. The protein specifically deubiquitinates a protein in the Fanconi anemia (FA) DNA repair pathway. Alternate transcriptional splice variants have been characterized. [provided by RefSeq, Jul 2008]
UniProt Code:	O94782
NCBI GenInfo Identifier:	12643657
NCBI Gene ID:	7398
NCBI Accession:	O94782.1
UniProt Secondary Accession:	O94782,Q05BX7, Q59H66, Q9UFR0, Q9UNJ3, A0PJ95, D3DQ57
UniProt Related Accession:	O94782
Molecular Weight:	785
NCBI Full Name:	Ubiquitin carboxyl-terminal hydrolase 1
NCBI Synonym Full Names:	ubiquitin specific peptidase 1
NCBI Official Symbol:	USP1
NCBI Official Synonym Symbols:	UBP
NCBI Protein Information:	ubiquitin carboxyl-terminal hydrolase 1; hUBP; ubiquitin thioesterase 1; deubiquitinating enzyme 1; ubiquitin thiolesterase 1; ubiquitin specific protease 1; ubiquitin carboxyl terminal hydrolase 1; ubiquitin specific processing protease 1; ubiquitin-specific-processing protease 1
UniProt Protein Name:	Ubiquitin carboxyl-terminal hydrolase 1
UniProt Synonym Protein Names:	Deubiquitinating enzyme 1; hUBP; Ubiquitin thioesterase 1; Ubiquitin-specific-processing protease 1
Protein Family:	UDP-sugar pyrophosphorylase
UniProt Gene Name:	USP1
UniProt Entry Name:	UBP1_HUMAN

Western blot analysis of extracts of various cell lines, using USP1 antibody (CAB6785) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.

Antibodies

USP1 Antibody (PACO13114)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)