Anti-UPF3B Antibody (CAB15184)
- SKU:
- CAB15184
- Product type:
- Antibody
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Frequently bought together:
Description
| Antibody Name: | Anti-UPF3B Antibody |
| Antibody SKU: | CAB15184 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human UPF3B (NP_075386.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Mouse |
| Positive Samples: | Mouse testis, Mouse heart |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human UPF3B (NP_075386.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MKEE KEHR PKEK RVTL LTPA GATG SGGG TSGD SSKG EDKQ DRNK EKKE ALSK VVIR RLPP TLTK EQLQ EHLQ PMPE HDYF EFFS NDTS LYPH MYAR AYIN FKNQ EDII LFRD RFDG YVFL DNKG QEYP AIVE FAPF QKAA KKKT KKRD TKVG TIDD DPEY RKFL ESYA TDNE KMTS TPET |
| Gene ID: | 65109 |
| Uniprot: | Q9BZI7 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 56kDa/57kDa |
| Observed MW: | 56kDa |
| Synonyms: | UPF3B, HUPF3B, MRX62, MRXS14, RENT3B, UPF3BP1, UPF3BP2, UPF3BP3, UPF3X, Upf3p-X |
| Background: | This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. The encoded protein is one of two functional homologs to yeast Upf3p. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein binds to the mRNA and remains bound after nuclear export, acting as a nucleocytoplasmic shuttling protein. It forms with Y14 a complex that binds specifically 20 nt upstream of exon-exon junctions. This gene is located on the long arm of chromosome X. Two splice variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | UPF3B: Involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by associating with the nuclear exon junction complex (EJC) and serving as link between the EJC core and NMD machinery. Recruits UPF2 at the cytoplasmic side of the nuclear envelope and the subsequent formation of an UPF1-UPF2- UPF3 surveillance complex (including UPF1 bound to release factors at the stalled ribosome) is believed to activate NMD. In cooperation with UPF2 stimulates both ATPase and RNA helicase activities of UPF1. Binds spliced mRNA upstream of exon-exon junctions. In vitro, stimulates translation; the function is indepenedent of association with UPF2 and components of the EJC core. Defects in UPF3B are the cause of mental retardation syndromic X-linked type 14 (MRXS14). Mental retardation is characterized by significantly below average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. MRXS14 patients manifest mental retardation associated with other variable signs such as autistic features, slender build, poor musculature, long, thin face, high-arched palate, high nasal bridge, and pectus deformities. Belongs to the RENT3 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:RNA splicing; RNA-binding Chromosomal Location of Human Ortholog: Xq24 Cellular Component: cytoplasm; cytosol; microtubule organizing center; nucleolus; nucleoplasm; nucleus Molecular Function:mRNA binding; nucleocytoplasmic transporter activity; nucleotide binding; protein binding Biological Process: mRNA 3'-end processing; mRNA catabolic process, nonsense-mediated decay; mRNA export from nucleus; nuclear mRNA splicing, via spliceosome; positive regulation of translation; RNA export from nucleus; termination of RNA polymerase II transcription Disease: Mental Retardation, X-linked, Syndromic 14 |
| NCBI Summary: | This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. The encoded protein is one of two functional homologs to yeast Upf3p. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein binds to the mRNA and remains bound after nuclear export, acting as a nucleocytoplasmic shuttling protein. It forms with Y14 a complex that binds specifically 20 nt upstream of exon-exon junctions. This gene is located on the long arm of chromosome X. Two splice variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9BZI7 |
| NCBI GenInfo Identifier: | 111309183 |
| NCBI Gene ID: | 65109 |
| NCBI Accession: | AAI21019.1 |
| UniProt Secondary Accession: | Q9BZI7,Q0VAK8, Q9H1J0, D3DWI3, D3DWI4, |
| UniProt Related Accession: | Q9BZI7 |
| Molecular Weight: | 56,213 Da |
| NCBI Full Name: | UPF3B protein |
| NCBI Synonym Full Names: | UPF3 regulator of nonsense transcripts homolog B (yeast) |
| NCBI Official Symbol: | UPF3B |
| NCBI Official Synonym Symbols: | MRX62; UPF3X; HUPF3B; MRXS14; RENT3B; UPF3BP1; UPF3BP2; UPF3BP3; Upf3p-X |
| NCBI Protein Information: | regulator of nonsense transcripts 3B |
| UniProt Protein Name: | Regulator of nonsense transcripts 3B |
| UniProt Synonym Protein Names: | Nonsense mRNA reducing factor 3B; Up-frameshift suppressor 3 homolog B; hUpf3B; Up-frameshift suppressor 3 homolog on chromosome X; hUpf3p-X |
| Protein Family: | Regulator of nonsense transcripts |
| UniProt Gene Name: | UPF3B |
| UniProt Entry Name: | REN3B_HUMAN |
 | Western blot analysis of extracts of various cell lines, using UPF3B antibody (CAB15184) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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