Anti-UNC5B Antibody (CAB14978)
- SKU:
- CAB14978
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cardiovascular
Frequently bought together:
Description
| Antibody Name: | Anti-UNC5B Antibody |
| Antibody SKU: | CAB14978 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 290-377 of human UNC5B (NP_734465.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-87MG, A-549, 293T, Mouse brain, Mouse lung, Mouse kidney, Rat brain, Rat lung |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 290-377 of human UNC5B (NP_734465.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | FQKT ACTT ICPV DGAW TEWS KWSA CSTE CAHW RSRE CMAP PPQN GGRD CSGT LLDS KNCT DGLC MQNK KTLS DPNS HLLE ASGD AALY |
| Gene ID: | 219699 |
| Uniprot: | Q8IZJ1 |
| Cellular Location: | Cell membrane, Membrane raft, Single-pass type I membrane protein |
| Calculated MW: | 102kDa/103kDa |
| Observed MW: | 104kDa |
| Synonyms: | UNC5B, UNC5H2, p53RDL1 |
| Background: | This gene encodes a member of the netrin family of receptors. This particular protein mediates the repulsive effect of netrin-1 and is a vascular netrin receptor. This encoded protein is also in a group of proteins called dependence receptors (DpRs) which are involved in pro- and anti-apoptotic processes. Many DpRs are involved in embryogenesis and in cancer progression. Two alternatively spliced transcript variants have been described for this gene. |
| UniProt Protein Function: | UNC5B: Receptor for netrin required for axon guidance. Mediates axon repulsion of neuronal growth cones in the developing nervous system upon ligand binding. Axon repulsion in growth cones may be caused by its association with DCC that may trigger signaling for repulsion. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand. Mediates apoptosis by activating DAPK1. In the absence of NTN1, activates DAPK1 by reducing its autoinhibitory phosphorylation at Ser-308 thereby increasing its catalytic activity. Belongs to the unc-5 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Membrane protein, integral; Receptor, misc. Chromosomal Location of Human Ortholog: 10q22.1 Molecular Function:protein binding Biological Process: negative regulation of neuron apoptosis; positive regulation of phosphoinositide 3-kinase cascade |
| NCBI Summary: | This gene encodes a member of the netrin family of receptors. This particular protein mediates the repulsive effect of netrin-1 and is a vascular netrin receptor. This encoded protein is also in a group of proteins called dependence receptors (DpRs) which are involved in pro- and anti-apoptotic processes. Many DpRs are involved in embryogenesis and in cancer progression. Two alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Oct 2011] |
| UniProt Code: | Q8IZJ1 |
| NCBI GenInfo Identifier: | 54036589 |
| NCBI Gene ID: | 219699 |
| NCBI Accession: | Q8IZJ1.2 |
| UniProt Secondary Accession: | Q8IZJ1,Q5T3R9, Q5T3S0, Q86SN3, Q8N1Y2, Q9H9F3, |
| UniProt Related Accession: | Q8IZJ1 |
| Molecular Weight: | 102,434 Da |
| NCBI Full Name: | Netrin receptor UNC5B |
| NCBI Synonym Full Names: | unc-5 netrin receptor B |
| NCBI Official Symbol: | UNC5B |
| NCBI Official Synonym Symbols: | UNC5H2; p53RDL1 |
| NCBI Protein Information: | netrin receptor UNC5B |
| UniProt Protein Name: | Netrin receptor UNC5B |
| UniProt Synonym Protein Names: | Protein unc-5 homolog 2; Protein unc-5 homolog B; p53-regulated receptor for death and life protein 1 |
| Protein Family: | Netrin receptor |
| UniProt Gene Name: | UNC5B |
| UniProt Entry Name: | UNC5B_HUMAN |
 | Western blot analysis of extracts of various cell lines, using UNC5B antibody (CAB14978) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
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