Anti-UNC13B Antibody (CAB15780)
- SKU:
- CAB15780
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-UNC13B Antibody |
| Antibody SKU: | CAB15780 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-350 of human UNC13B (NP_006368.3). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | 293T, LO2 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-350 of human UNC13B (NP_006368.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MSLL CVRV KRAK FQGS PDKF NTYV TLKV QNVK STTV AVRG DQPS WEQD FMFE ISRL DLGL SVEV WNKG LIWD TMVG TVWI ALKT IRQS DEEG PGEW STLE AETL MKDD EICG TRNP TPHK ILLD TRFE LPFD IPEE EARY WTYK WEQI NALG ADNE YSSQ EESQ RKPL PTAA AQCS FEDP DSAV DDRD SDYR SETS NSFP PPYH TASQ PNAS VHQF PVPV RSPQ QLLL QGSS RDSC NDSM QSYD LDYP ERRA ISPT SSSR YGSS CNVS QGSS QLSE LDQY HEQD DDHR ETDS IHSC HSSH SLSR DGQA GFGE QEKP LEVT GQAE KEAA CEPK EMKE DATT HPPP DLVL QK |
| Gene ID: | 10497 |
| Uniprot: | O14795 |
| Cellular Location: | Cell junction, Cell membrane, Cytoplasm, Membrane, Peripheral membrane protein, synapse |
| Calculated MW: | 180kDa/182kDa |
| Observed MW: | 210kDa |
| Synonyms: | UNC13B, MUNC13, UNC13, Unc13h2 |
| Background: | This gene is expressed in the kidney cortical epithelial cells and is upregulated by hyperglycemia. The encoded protein shares a high level of similarity to the rat homolog, and contains 3 C2 domains and a diacylglycerol-binding C1 domain. Hyperglycemia increases the levels of diacylglycerol, which has been shown to induce apoptosis in cells transfected with this gene and thus contribute to the renal cell complications of hyperglycemia. Studies in other species also indicate a role for this protein in the priming step of synaptic vesicle exocytosis. |
| UniProt Protein Function: | UNC13B: Plays a role in vesicle maturation during exocytosis as a target of the diacylglycerol second messenger pathway. Is involved in neurotransmitter release by acting in synaptic vesicle priming prior to vesicle fusion and participates in the activity- depending refilling of readily releasable vesicle pool (RRP). Essential for synaptic vesicle maturation in a subset of excitatory/glutamatergic but not inhibitory/GABA-mediated synapses. Belongs to the unc-13 family. |
| UniProt Protein Details: | Protein type:Lipid-binding Chromosomal Location of Human Ortholog: 9p13.3 Cellular Component: Golgi apparatus; cytoplasm; presynaptic active zone; plasma membrane; terminal button; neuromuscular junction; cytosol; cell junction Molecular Function:diacylglycerol binding; protein binding; signal transducer activity; metal ion binding; receptor activity Biological Process: synaptic transmission; synaptic transmission, glutamatergic; glutamate secretion; apoptosis; neurotransmitter secretion; regulation of short-term neuronal synaptic plasticity; excretion; signal transduction; synaptic vesicle priming; neuromuscular junction development; synaptic vesicle docking during exocytosis |
| NCBI Summary: | This gene is expressed in the kidney cortical epithelial cells and is upregulated by hyperglycemia. The encoded protein shares a high level of similarity to the rat homolog, and contains 3 C2 domains and a diacylglycerol-binding C1 domain. Hyperglycemia increases the levels of diacylglycerol, which has been shown to induce apoptosis in cells transfected with this gene and thus contribute to the renal cell complications of hyperglycemia. Studies in other species also indicate a role for this protein in the priming step of synaptic vesicle exocytosis. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O14795 |
| NCBI GenInfo Identifier: | 2432000 |
| NCBI Gene ID: | 10497 |
| NCBI Accession: | AAC19406.1 |
| UniProt Secondary Accession: | O14795,Q5VYM8, |
| UniProt Related Accession: | O14795 |
| Molecular Weight: | 180,679 Da |
| NCBI Full Name: | Munc13 |
| NCBI Synonym Full Names: | unc-13 homolog B (C. elegans) |
| NCBI Official Symbol: | UNC13B |
| NCBI Official Synonym Symbols: | UNC13; MUNC13; Unc13h2 |
| NCBI Protein Information: | protein unc-13 homolog B; munc13-2; homolog of rat Munc13 (diacylglycerol-binding) |
| UniProt Protein Name: | Protein unc-13 homolog B |
| UniProt Synonym Protein Names: | Munc13-2 |
| Protein Family: | Protein |
| UniProt Gene Name: | UNC13B |
| UniProt Entry Name: | UN13B_HUMAN |
 | Western blot analysis of extracts of various cell lines, using UNC13B antibody (CAB15780) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunohistochemistry of paraffin-embedded Human liver using UNC13B Rabbit pAb (CAB15780) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of L929 cells using UNC13B antibody (CAB15780) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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