Anti-ULK2 항체 (CAB15243)

Antibody Name:	Anti-ULK2 Antibody
Antibody SKU:	CAB15243
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ULK2 (NP_001136082.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Rat
Positive Samples:	PC-3

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ULK2 (NP_001136082.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MEVV GDFE YSKR DLVG HGAF AVVF RGRH RQKT DWEV AIKS INKK NLSK SQIL LGKE IKIL KELQ HENI VALY DVQE LPNS VFLV MEYC NGGD LADY LQAK
Gene ID:	9706
Uniprot:	Q8IYT8
Cellular Location:	Cytoplasmic vesicle membrane, Peripheral membrane protein
Calculated MW:	112kDa
Observed MW:	113kDa

Synonyms:

ULK2, ATG1B, Unc51.2

Background:

This gene encodes a protein that is similar to a serine/threonine kinase in C. elegans which is involved in axonal elongation. The structure of this protein is similar to the C. elegans protein in that both proteins have an N-terminal kinase domain, a central proline/serine rich (PS) domain, and a C-terminal (C) domain. The gene is located within the Smith-Magenis syndrome region on chromosome 17. Alternatively spliced transcript variants encoding the same protein have been identified.

UniProt Protein Function:	ULK2: Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3- kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and a negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK, also acts as a negative regulator of AMPK through phosphorylation of the AMPK subunits PRKAA1, PRKAB2 and PRKAG1. May phosphorylate ATG13/KIAA0652, FRS2, FRS3 and RPTOR; however such data need additional evidences. Not involved in ammonia-induced autophagy or in autophagic response of cerebellar granule neurons (CGN) to low potassium concentration. Plays a role early in neuronal differentiation and is required for granule cell axon formation: may govern axon formation via Ras-like GTPase signaling and through regulation of the Rab5-mediated endocytic pathways within developing axons. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily.
UniProt Protein Details:	Protein type:Kinase, protein; Autophagy; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Protein kinase, Other; Other group; ULK family Chromosomal Location of Human Ortholog: 17p11.2 Cellular Component: pre-autophagosomal structure membrane Molecular Function:protein binding; protein serine/threonine kinase activity Biological Process: axon extension; protein amino acid autophosphorylation; regulation of autophagy; response to starvation
NCBI Summary:	This gene encodes a protein that is similar to a serine/threonine kinase in C. elegans which is involved in axonal elongation. The structure of this protein is similar to the C. elegans protein in that both proteins have an N-terminal kinase domain, a central proline/serine rich (PS) domain, and a C-terminal (C) domain. The gene is located within the Smith-Magenis syndrome region on chromosome 17. Alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq, Dec 2008]
UniProt Code:	Q8IYT8
NCBI GenInfo Identifier:	296453001
NCBI Gene ID:	9706
NCBI Accession:	Q8IYT8.3
UniProt Secondary Accession:	Q8IYT8,O75119, A8MY69,
UniProt Related Accession:	Q8IYT8
Molecular Weight:	112,694 Da
NCBI Full Name:	Serine/threonine-protein kinase ULK2
NCBI Synonym Full Names:	unc-51 like autophagy activating kinase 2
NCBI Official Symbol:	ULK2
NCBI Official Synonym Symbols:	ATG1B; Unc51.2
NCBI Protein Information:	serine/threonine-protein kinase ULK2
UniProt Protein Name:	Serine/threonine-protein kinase ULK2
UniProt Synonym Protein Names:	Unc-51-like kinase 2
Protein Family:	Serine/threonine-protein kinase
UniProt Gene Name:	ULK2
UniProt Entry Name:	ULK2_HUMAN

Western blot analysis of extracts of PC-3 cells, using ULK2 antibody (CAB15243) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.

Antibodies

Anti-ULK2 Antibody (CAB15244)

ULK2 Antibody (PACO07261)

ULK2 Antibody (PACO07279)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)