Anti-ULk1 Antibody (CAB8529)
- SKU:
- CAB8529
- Product type:
- Antibody
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Autophagy
Frequently bought together:
Description
| Antibody Name: | Anti-ULk1 Antibody |
| Antibody SKU: | CAB8529 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 400-499 of human ULk1 (NP_003556.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:1000 IHC 1:50 - 1:200 IF 1:10 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 293T, HeLa |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 400-499 of human ULk1 (NP_003556.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | RTPS PSPP CSSS PSPS GRAG PFSS SRCG ASVP IPVP TQVQ NYQR IERN LQSP TQFQ TPRS SAIR RSGS TSPL GFAR ASPS PPAH AEHG GVLA RKMS LGGG |
| Gene ID: | 8408 |
| Uniprot: | O75385 |
| Cellular Location: | Cytoplasm, Preautophagosomal structure, cytosol |
| Calculated MW: | 112kDa |
| Observed MW: | 150kDa |
| Synonyms: | ULK1, ATG1, ATG1A, UNC51, Unc51.1, hATG1, ULk1 |
| Background: |
| UniProt Protein Function: | ULK1: Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3- kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation. Interacts with GABARAP and GABARAPL2. Interacts (via C- terminus) with ATG13/KIAA0652. Part of a complex consisting of ATG13/KIAA0652, ULK1 and RB1CC1. Associates with the mammalian target of rapamycin complex 1 (mTORC1) through an interaction with RPTOR; the association depends on nutrient conditions and is reduced during starvation. Ubiquitously expressed. Detected in the following adult tissues: skeletal muscle, heart, pancreas, brain, placenta, liver, kidney, and lung. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily. |
| UniProt Protein Details: | Protein type:Protein kinase, Other; Autophagy; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Other group; ULK family Chromosomal Location of Human Ortholog: 12q24.3 Cellular Component: autophagic vacuole; cytosol; pre-autophagosomal structure membrane Molecular Function:protein binding; protein complex binding; protein serine/threonine kinase activity; Rab GTPase binding Biological Process: axon extension; cellular response to nutrient levels; macroautophagy; negative regulation of protein complex assembly; neurite development; peptidyl-serine phosphorylation; positive regulation of autophagy; positive regulation of macroautophagy; protein amino acid autophosphorylation; protein localization; regulation of autophagy; response to starvation |
| UniProt Code: | O75385 |
| NCBI GenInfo Identifier: | 317373288 |
| NCBI Gene ID: | 8408 |
| NCBI Accession: | O75385.2 |
| UniProt Secondary Accession: | O75385,Q9UQ28, |
| UniProt Related Accession: | O75385 |
| Molecular Weight: | 112,631 Da |
| NCBI Full Name: | Serine/threonine-protein kinase ULK1 |
| NCBI Synonym Full Names: | unc-51 like autophagy activating kinase 1 |
| NCBI Official Symbol: | ULK1 |
| NCBI Official Synonym Symbols: | ATG1; ATG1A; UNC51; hATG1; Unc51.1 |
| NCBI Protein Information: | serine/threonine-protein kinase ULK1 |
| UniProt Protein Name: | Serine/threonine-protein kinase ULK1 |
| UniProt Synonym Protein Names: | Autophagy-related protein 1 homolog; ATG1; hATG1; Unc-51-like kinase 1 |
| Protein Family: | Serine/threonine-protein kinase |
| UniProt Gene Name: | ULK1 |
| UniProt Entry Name: | ULK1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ULK1 antibody (CAB8529) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s. |
 | Immunohistochemistry of paraffin-embedded mouse liver using ULk1 antibody (CAB8529) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse heart using ULk1 antibody (CAB8529) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of L929 cells using ULK1 antibody (CAB8529) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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