Anti-UIMC1 Antibody (CAB14512)
- SKU:
- CAB14512
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Frequently bought together:
Description
| Antibody Name: | Anti-UIMC1 Antibody |
| Antibody SKU: | CAB14512 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 449-719 of human UIMC1 (NP_057374.3). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | DU145, 293T, HeLa, Mouse thymus |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 449-719 of human UIMC1 (NP_057374.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | QLSS SETF DLER EVSP GSRD ILDG VRII MADK EVGN KEDA EKEV AIST FSSS NQVS CPLC DQCF PPTK IERH AMYC NGLM EEDT VLTR RQKE AKTK SDSG TAAQ TSLD IDKN EKCY LCKS LVPF REYQ CHVD SCLQ LAKA DQGD GPEG SGRA CSTV EGKW QQRL KNPK EKGH SEGR LLSF LEQS EHKT SDAD IKSS ETGA FRVP SPGM EEAG CSRE MQSS FTRR DLNE SPVK SFVS ISEA TDCL VDFK KQVT VQPG SRTR TKAG RGRR RKF |
| Gene ID: | 51720 |
| Uniprot: | Q96RL1 |
| Cellular Location: | Nucleus |
| Calculated MW: | 17kDa/38kDa/61kDa/70kDa/79kDa |
| Observed MW: | 102kDa |
| Synonyms: | UIMC1, RAP80, X2HRIP110 |
| Background: | This gene encodes a nuclear protein that interacts with Brca1 (breast cancer 1) in a complex to recognize and repair DNA lesions. This protein binds ubiquitinated lysine 63 of histone H2A and H2AX. This protein may also function as a repressor of transcription. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | RAP80: Ubiquitin-binding protein that specifically recognizes and binds 'Lys-63'-linked ubiquitin. Plays a central role in the BRCA1-A complex by specifically binding 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. Also weakly binds monoubiquitin but with much less affinity than 'Lys-63'-linked ubiquitin. May interact with monoubiquitinated histones H2A and H2B; the relevance of such results is however unclear in vivo. Does not bind Lys-48'-linked ubiquitin. May indirectly Act as a transcriptional repressor by inhibiting the interaction of NR6A1 with the corepressor NCOR1. Interacts with TSP57. Component of the BRCA1-A complex, at least composed of the BRCA1, BARD1, UIMC1/RAP80, FAM175A/Abraxas, BRCC3/BRCC36, BRE/BRCC45 and BABAM1/NBA1. In the BRCA1-A complex, interacts directly with FAM175A/Abraxas. Interacts with ESR1, NR6A1 and UBE2I. Expressed in testis, ovary, thymus and heart. Expressed in germ cells of the testis. Belongs to the RAP80 family. 5 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Transcription, coactivator/corepressor; Nuclear receptor co-regulator Chromosomal Location of Human Ortholog: 5q35.2 Cellular Component: nucleus Molecular Function:retinoid X receptor binding; protein binding; histone binding Biological Process: positive regulation of DNA repair; transcription, DNA-dependent; double-strand break repair; response to ionizing radiation; negative regulation of transcription, DNA-dependent; G2/M transition DNA damage checkpoint |
| UniProt Code: | Q96RL1 |
| NCBI GenInfo Identifier: | 60390957 |
| NCBI Gene ID: | 51720 |
| NCBI Accession: | Q96RL1.2 |
| UniProt Secondary Accession: | Q96RL1,Q5XKQ1, Q7Z3W7, Q8N5B9, Q9BZR1, Q9BZR5, Q9UHX7 A8MSA1, B3KMZ1, B4E3N2, |
| UniProt Related Accession: | Q96RL1 |
| Molecular Weight: | 719 |
| NCBI Full Name: | BRCA1-A complex subunit RAP80 |
| NCBI Synonym Full Names: | ubiquitin interaction motif containing 1 |
| NCBI Official Symbol: | UIMC1 |
| NCBI Official Synonym Symbols: | RAP80; X2HRIP110 |
| NCBI Protein Information: | BRCA1-A complex subunit RAP80; receptor associated protein 80; receptor-associated protein 80; retinoid x receptor interacting protein; retinoid X receptor-interacting protein 110; ubiquitin interaction motif-containing protein 1 |
| UniProt Protein Name: | BRCA1-A complex subunit RAP80 |
| UniProt Synonym Protein Names: | Receptor-associated protein 80; Retinoid X receptor-interacting protein 110; Ubiquitin interaction motif-containing protein 1 |
| Protein Family: | BRCA1-A complex |
| UniProt Gene Name: | UIMC1 |
| UniProt Entry Name: | UIMC1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using UIMC1 antibody (CAB14512) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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