Anti-UCP1 항체 (CAB5857)

Antibody Name:	Anti-UCP1 Antibody
Antibody SKU:	CAB5857
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-307 of human UCP1 (NP_068605.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse kidney, HL-60, Rat lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-307 of human UCP1 (NP_068605.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MGGL TASD VHPT LGVQ LFSA GIAA CLAD VITF PLDT AKVR LQVQ GECP TSSV IRYK GVLG TITA VVKT EGRM KLYS GLPA GLQR QISS ASLR IGLY DTVQ EFLT AGKE TAPS LGSK ILAG LTTG GVAV FIGQ PTEV VKVR LQAQ SHLH GIKP RYTG TYNA YRII ATTE GLTG LWKG TTPN LMRS VIIN CTEL VTYD LMKE AFVK NNIL ADDV PCHL VSAL IAGF CATA MSSP VDVV KTRF INSP PGQY KSVP NCAM KVFT NEGP TAFF KGLV PSFL RLGS WNVI MFVC FEQL KREL SKSR QTMD CAT
Gene ID:	7350
Uniprot:	P25874
Cellular Location:	Mitochondrion inner membrane, Multi-pass membrane protein
Calculated MW:	33kDa
Observed MW:	33kDa

Synonyms:

UCP1, SLC25A7, UCP

Background:

Mitochondrial uncoupling proteins (UCP) are members of the family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. Tissue specificity occurs for the different UCPs and the exact methods of how UCPs transfer H+/OH- are not known. UCPs contain the three homologous protein domains of MACPs. This gene is expressed only in brown adipose tissue, a specialized tissue which functions to produce heat.

UniProt Protein Function:	UCP1: UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat. Belongs to the mitochondrial carrier family.
UniProt Protein Details:	Protein type:Membrane protein, multi-pass; Membrane protein, integral; Mitochondrial Chromosomal Location of Human Ortholog: 4q28-q31 Cellular Component: mitochondrion; mitochondrial inner membrane; integral to membrane Molecular Function:oxidative phosphorylation uncoupler activity Biological Process: regulation of transcription from RNA polymerase II promoter; cellular metabolic process; proton transport; brown fat cell differentiation; mitochondrial transport Disease: Obesity
NCBI Summary:	Mitochondrial uncoupling proteins (UCP) are members of the family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. Tissue specificity occurs for the different UCPs and the exact methods of how UCPs transfer H+/OH- are not known. UCPs contain the three homologous protein domains of MACPs. This gene is expressed only in brown adipose tissue, a specialized tissue which functions to produce heat. [provided by RefSeq, Jul 2008]
UniProt Code:	P25874
NCBI GenInfo Identifier:	71153184
NCBI Gene ID:	7350
NCBI Accession:	P25874.3
UniProt Secondary Accession:	P25874,Q13218, Q4KMZ3, Q68G66,
UniProt Related Accession:	P25874
Molecular Weight:	307
NCBI Full Name:	Mitochondrial brown fat uncoupling protein 1
NCBI Synonym Full Names:	uncoupling protein 1 (mitochondrial, proton carrier)
NCBI Official Symbol:	UCP1
NCBI Official Synonym Symbols:	UCP; SLC25A7
NCBI Protein Information:	mitochondrial brown fat uncoupling protein 1; thermogenin; solute carrier family 25 member 7
UniProt Protein Name:	Mitochondrial brown fat uncoupling protein 1
UniProt Synonym Protein Names:	Solute carrier family 25 member 7; Thermogenin
Protein Family:	Mitochondrial brown fat uncoupling protein
UniProt Gene Name:	UCP1
UniProt Entry Name:	UCP1_HUMAN

	Western blot analysis of extracts of various cell lines, using UCP1 antibody (CAB5857) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Western blot analysis of extracts of Rat lung, using UCP1 antibody (CAB5857) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3min.
	Immunohistochemistry of paraffin-embedded Rat fat using UCP1 antibody (CAB5857) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of L929 cells using UCP1 antibody (CAB5857) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-UCP1 Antibody (CAB7236)	Human UCP1 ELISA Kit
	Human Mitochondrial brown fat uncoupling protein 1 (UCP1) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)