Anti-Ubiquilin-2 Antibody (CAB9568)
- SKU:
- CAB9568
- Product type:
- Antibody
- Application:
- WB
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Autophagy
Frequently bought together:
Description
| Antibody Name: | Anti-Ubiquilin-2 Antibody |
| Antibody SKU: | CAB9568 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human UBQLN2 (NP_038472.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:200 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | B cells, NCI-H460, Mouse brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human UBQLN2 (NP_038472.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAEN GESS GPPR PSRG PAAA QGSA AAPA EPKI IKVT VKTP KEKE EFAV PENS SVQQ FKEA ISKR FKSQ TDQL VLIF AGKI LKDQ DTLI QHGI HDGL TVHL VIKS QNRP QGQS TQPS NAAG TNTT SAST PRSN STPI STNS NPFG LGSL GG |
| Gene ID: | 29978 |
| Uniprot: | Q9UHD9 |
| Cellular Location: | Cytoplasm, Cytoplasmic vesicle, Membrane, Nucleus, autophagosome |
| Calculated MW: | 65kDa |
| Observed MW: | 70kDa |
| Synonyms: | UBQLN2, ALS15, CHAP1, DSK2, HRIHFB2157, N4BP4, PLIC2 |
| Background: | This gene encodes an ubiquitin-like protein (ubiquilin) that shares high degree of similarity with related products in yeast, rat and frog. Ubiquilins contain a N-terminal ubiquitin-like domain and a C-terminal ubiquitin-associated domain. They physically associate with both proteasomes and ubiquitin ligases; and thus, are thought to functionally link the ubiquitination machinery to the proteasome to affect in vivo protein degradation. This ubiquilin has also been shown to bind the ATPase domain of the Hsp70-like Stch protein. |
| UniProt Protein Function: | UBQLN2: Increases the half-life of proteins destined to be degraded by the proteasome; may modulate proteasome-mediated protein degradation. Defects in UBQLN2 are the cause of amyotrophic lateral sclerosis type 15 with or without frontotemporal dementia (ALS15). A neurodegenerative disorder affecting upper motor neurons in the brain and lower motor neurons in the brain stem and spinal cord, resulting in fatal paralysis. Sensory abnormalities are absent. The pathologic hallmarks of the disease include pallor of the corticospinal tract due to loss of motor neurons, presence of ubiquitin-positive inclusions within surviving motor neurons, and deposition of pathologic aggregates. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of the cases. Patients with ALS15 may develop frontotemporal dementia. |
| UniProt Protein Details: | Protein type:Ubiquitin conjugating system Chromosomal Location of Human Ortholog: Xp11.21 Cellular Component: cytoplasm; plasma membrane Molecular Function:protein binding Biological Process: ER-associated protein catabolic process; regulation of macroautophagy Disease: Amyotrophic Lateral Sclerosis 15, With Or Without Frontotemporal Dementia |
| NCBI Summary: | This gene encodes an ubiquitin-like protein (ubiquilin) that shares high degree of similarity with related products in yeast, rat and frog. Ubiquilins contain a N-terminal ubiquitin-like domain and a C-terminal ubiquitin-associated domain. They physically associate with both proteasomes and ubiquitin ligases; and thus, are thought to functionally link the ubiquitination machinery to the proteasome to affect in vivo protein degradation. This ubiquilin has also been shown to bind the ATPase domain of the Hsp70-like Stch protein. [provided by RefSeq, Oct 2009] |
| UniProt Code: | Q9UHD9 |
| NCBI GenInfo Identifier: | 124056593 |
| NCBI Gene ID: | 29978 |
| NCBI Accession: | Q9UHD9.2 |
| UniProt Secondary Accession: | Q9UHD9,O94798, Q5D027, Q9H3W6, Q9HAZ4, |
| UniProt Related Accession: | Q9UHD9 |
| Molecular Weight: | 65,696 Da |
| NCBI Full Name: | Ubiquilin-2 |
| NCBI Synonym Full Names: | ubiquilin 2 |
| NCBI Official Symbol: | UBQLN2 |
| NCBI Official Synonym Symbols: | DSK2; ALS15; CHAP1; N4BP4; PLIC2; HRIHFB2157 |
| NCBI Protein Information: | ubiquilin-2 |
| UniProt Protein Name: | Ubiquilin-2 |
| UniProt Synonym Protein Names: | Chap1; DSK2 homolog; Protein linking IAP with cytoskeleton 2; PLIC-2; hPLIC-2; Ubiquitin-like product Chap1/Dsk2 |
| Protein Family: | Ubiquilin |
| UniProt Gene Name: | UBQLN2 |
| UniProt Entry Name: | UBQL2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using UBQLN2 antibody (CAB9568) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of HeLa cells using UBQLN2 Polyclonal Antibody (CAB9568) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of NIH-3T3 cells using UBQLN2 Polyclonal Antibody (CAB9568) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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