Anti-UBE2W Antibody (CAB16691)

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SKU:
CAB16691
Product type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
Frequently bought together:

Description

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Antibody Name:Anti-UBE2W Antibody
Antibody SKU:CAB16691
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 40-110 of human UBE2W (NP_001001481.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:1000
Reactivity:Human, Mouse
Positive Samples:MCF7, LO2, BxPC-3, mouse testis
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 40-110 of human UBE2W (NP_001001481.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, pH7.3.
Isotype:IgG
Sequence:VEVW FPKR LQKE LLAL QNDP PPGM TLNE KSVQ NSIT QWIV DMEG APGT LYEG EKFQ LLFK FSSR YPFD SPQ
Gene ID:55284
Uniprot:Q96B02
Cellular Location:Nucleus
Calculated MW:17kDa/18kDa/20kDa
Observed MW:17kDa
Synonyms:UBE2W, UBC-16, UBC16
Background:This gene encodes a nuclear-localized ubiquitin-conjugating enzyme (E2) that, along with ubiquitin-activating (E1) and ligating (E3) enzymes, coordinates the addition of a ubiquitin moiety to existing proteins. The encoded protein promotes the ubiquitination of Fanconi anemia complementation group proteins and may be important in the repair of DNA damage. There is a pseudogene for this gene on chromosome 1. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:UBE2W: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes monoubiquitination. Involved in degradation of misfolded chaperone substrates by mediating monoubiquitination of STUB1/CHIP, leading to recruitment of ATXN3 to monoubiquitinated STUB1/CHIP, and restriction of the length of ubiquitin chain attached to STUB1/CHIP substrates by ATXN3. After UV irradiation, but not after mitomycin-C (MMC) treatment, acts as a specific E2 ubiquitin-conjugating enzyme for the Fanconi anemia complex by associating with E3 ubiquitin-protein ligase FANCL and catalyzing monoubiquitination of FANCD2, a key step in the DNA damage pathway. In vitro catalyzes 'Lys-11'-linked polyubiquitination. Belongs to the ubiquitin-conjugating enzyme family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Ubiquitin ligase; Ubiquitin conjugating system; EC 6.3.2.19; Enzyme, misc.; Ligase

Chromosomal Location of Human Ortholog: 8q21.11

Cellular Component: cytoplasm; nucleus

Molecular Function:protein binding; ubiquitin protein ligase binding; ubiquitin-protein ligase activity

Biological Process: misfolded or incompletely synthesized protein catabolic process; proteasomal ubiquitin-dependent protein catabolic process; protein monoubiquitination

NCBI Summary:This gene encodes a nuclear-localized ubiquitin-conjugating enzyme (E2) that, along with ubiquitin-activating (E1) and ligating (E3) enzymes, coordinates the addition of a ubiquitin moiety to existing proteins. The encoded protein promotes the ubiquitination of Fanconi anemia complementation group proteins and may be important in the repair of DNA damage. There is a pseudogene for this gene on chromosome 1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]
UniProt Code:Q96B02
NCBI GenInfo Identifier:74751754
NCBI Gene ID:55284
NCBI Accession:Q96B02.1
UniProt Secondary Accession:Q96B02,Q1XBE0, Q9H823, Q9HAG6, Q9NV07, B4DIV1,
UniProt Related Accession:Q96B02
Molecular Weight:20,689 Da
NCBI Full Name:Ubiquitin-conjugating enzyme E2 W
NCBI Synonym Full Names:ubiquitin conjugating enzyme E2 W (putative)
NCBI Official Symbol:UBE2W  
NCBI Official Synonym Symbols:UBC16; UBC-16  
NCBI Protein Information:ubiquitin-conjugating enzyme E2 W
UniProt Protein Name:Ubiquitin-conjugating enzyme E2 W
UniProt Synonym Protein Names:E2 ubiquitin-conjugating enzyme W; N-terminal E2 ubiquitin-conjugating enzyme (EC:2.3.2.25
Protein Family:Ubiquitin-conjugating enzyme
UniProt Gene Name:UBE2W  
UniProt Entry Name:UBE2W_HUMAN
Anti-UBE2W Antibody (CAB16691)Western blot analysis of extracts of various cell lines, using UBE2W antibody (CAB16691) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

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