Anti-UBE2U Antibody (CAB8376)
- SKU:
- CAB8376
- Product type:
- Antibody
- Application:
- WB
- Application:
- IHC
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Signal Transduction
Frequently bought together:
Description
| Antibody Name: | Anti-UBE2U Antibody |
| Antibody SKU: | CAB8376 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-226 of human UBE2U (NP_689702.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 |
| Reactivity: | Human, Mouse |
| Positive Samples: | U-251MG, LO2, NCI-H460 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-226 of human UBE2U (NP_689702.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MHGR AYLL LHRD FCDL KENN YKGI TAKP VSED MMEW EVEI EGLQ NSVW QGLV FQLT IHFT SEYN YAPP VVKF ITIP FHPN VDPH TGQP CIDF LDNP EKWN TNYT LSSI LLAL QVML SNPV LENP VNLE AARI LVKD ESLY RTIL RLFN RPLQ MKDD SQEL PKDP RKCI RPIK TTSF SDYY QTWS RIAT SKAT EYYR TPLL KVPN FIGQ YYKW KKMD LQHQ KEWN LK |
| Gene ID: | 148581 |
| Uniprot: | Q5VVX9 |
| Cellular Location: | |
| Calculated MW: | 26kDa/37kDa |
| Observed MW: | 42kDa |
| Synonyms: | UBE2U |
| Background: |
| UniProt Protein Function: | UBE2U: Catalyzes the covalent attachment of ubiquitin to other proteins. Belongs to the ubiquitin-conjugating enzyme family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Ligase; EC 6.3.2.19; Ubiquitin ligase; Ubiquitin conjugating system Chromosomal Location of Human Ortholog: 1p31.3 Cellular Component: cytoplasm; nuclear chromatin Molecular Function:protein binding; ubiquitin protein ligase binding Biological Process: DNA repair; histone ubiquitination; proteasomal ubiquitin-dependent protein catabolic process; protein polyubiquitination |
| UniProt Code: | Q5VVX9 |
| NCBI GenInfo Identifier: | 74747311 |
| NCBI Gene ID: | 148581 |
| NCBI Accession: | Q5VVX9.1 |
| UniProt Secondary Accession: | Q5VVX9,Q8N1D4, |
| UniProt Related Accession: | Q5VVX9 |
| Molecular Weight: | 26,689 Da |
| NCBI Full Name: | Ubiquitin-conjugating enzyme E2 U |
| NCBI Synonym Full Names: | ubiquitin conjugating enzyme E2 U (putative) |
| NCBI Official Symbol: | UBE2U |
| NCBI Protein Information: | ubiquitin-conjugating enzyme E2 U |
| UniProt Protein Name: | Ubiquitin-conjugating enzyme E2 U |
| UniProt Synonym Protein Names: | E2 ubiquitin-conjugating enzyme U; Ubiquitin carrier protein U; Ubiquitin-protein ligase U |
| Protein Family: | Ubiquitin-conjugating enzyme |
| UniProt Gene Name: | UBE2U |
| UniProt Entry Name: | UBE2U_HUMAN |
 | Western blot analysis of extracts of various cell lines, using UBE2U antibody (CAB8376) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded human liver using UBE2U antibody (CAB8376) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse brain using UBE2U antibody (CAB8376) at dilution of 1:100 (40x lens). |
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