Anti-UBE2S Antibody (CAB18064)[KO Validated]

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SKU:
CAB18064
Product type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Cycle
Frequently bought together:

Description

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Antibody Name:Anti-UBE2S Antibody [KO Validated]
Antibody SKU:CAB18064
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 122-221 of human UBE2S (NP_055316.2).
Application:WB IHC
Recommended Dilution:WB 1:200 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:293T
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 122-221 of human UBE2S (NP_055316.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:HPNP ESAL NEEA GRLL LENY EEYA ARAR LLTE IHGG AGGP SGRA EAGR ALAS GTEA SSTD PGAP GGPG GAEG PMAK KHAG ERDK KLAA KKKT DKKR ALRR
Gene ID:27338
Uniprot:Q16763
Cellular Location:
Calculated MW:23kDa
Observed MW:26kDa
Anti-UBE2S Antibody [KO Validated] (CAB18064)Western blot analysis of extracts from normal (control) and UBE2S knockout (KO) 293T cells, using UBE2S antibody (CAB18064) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
Synonyms:UBE2S, E2-EPF, E2EPF, EPF5
Background:This gene encodes a member of the ubiquitin-conjugating enzyme family. The encoded protein is able to form a thiol ester linkage with ubiquitin in a ubiquitin activating enzyme-dependent manner, a characteristic property of ubiquitin carrier proteins.
UniProt Protein Function:UBE2S: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes 'Lys-11'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by specifically elongating 'Lys-11'-linked polyubiquitin chains initiated by the E2 enzyme UBE2C/UBCH10 on APC/C substrates, enhancing the degradation of APC/C substrates by the proteasome and promoting mitotic exit. Also acts by elongating ubiquitin chains initiated by the E2 enzyme UBE2D1/UBCH5 in vitro; it is however unclear whether UBE2D1/UBCH5 acts as a E2 enzyme for the APC/C in vivo. Also involved in ubiquitination and subsequent degradation of VHL, resulting in an accumulation of HIF1A. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, except 'Lys-48'-linked polyubiquitination. Component of the APC/C complex. Interacts with CDC20, FZR1/CDH1 and VHL. Belongs to the ubiquitin-conjugating enzyme family.
UniProt Protein Details:

Protein type:Ubiquitin ligase; EC 6.3.2.19; Ubiquitin conjugating system; Ligase

Chromosomal Location of Human Ortholog: 19q13.43

Cellular Component: anaphase-promoting complex; cytoplasm

Molecular Function:small conjugating protein ligase activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; ATP binding; ligase activity

Biological Process: anaphase-promoting complex activation; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; cell division; protein modification process; exit from mitosis

NCBI Summary:This gene encodes a member of the ubiquitin-conjugating enzyme family. The encoded protein is able to form a thiol ester linkage with ubiquitin in a ubiquitin activating enzyme-dependent manner, a characteristic property of ubiquitin carrier proteins. [provided by RefSeq, Jul 2008]
UniProt Code:Q16763
NCBI GenInfo Identifier:23829978
NCBI Gene ID:27338
NCBI Accession:Q16763.2
UniProt Related Accession:Q16763
Molecular Weight:
NCBI Full Name:Ubiquitin-conjugating enzyme E2 S
NCBI Synonym Full Names:ubiquitin conjugating enzyme E2 S
NCBI Official Symbol:UBE2S  
NCBI Official Synonym Symbols:EPF5; E2EPF; E2-EPF  
NCBI Protein Information:ubiquitin-conjugating enzyme E2 S
UniProt Protein Name:Ubiquitin-conjugating enzyme E2 S
UniProt Synonym Protein Names:E2-EPF; Ubiquitin carrier protein S; Ubiquitin-conjugating enzyme E2-24 kDa; Ubiquitin-conjugating enzyme E2-EPF5; Ubiquitin-protein ligase S
Protein Family:Ubiquitin-conjugating enzyme
UniProt Gene Name:UBE2S  
UniProt Entry Name:UBE2S_HUMAN

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