Anti-UBE2J2 Antibody (CAB7316)

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SKU:
CAB7316
Product type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction
Frequently bought together:

Description

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Antibody Name:Anti-UBE2J2 Antibody
Antibody SKU:CAB7316
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human UBE2J2 (NP_477515.2).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:U-251MG, HeLa, 293T, Mouse testis, Mouse brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human UBE2J2 (NP_477515.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MSST SSKR APTT ATQR LKQD YLRI KKDP VPYI CAEP LPSN ILEW HYVV RGPE MTPY EGGY YHGK LIFP REFP FKPP SIYM ITPN GRFK CNTR LCLS ITDF HPDT WNPA WSVS TILT GLLS FMVE KGPT LGSI ETSD FTKR QLAV QSLA FNLK DKVF CELF PEVV EEIK QKQK AQDE LSSR PQTL PLPD VVPD GETH LVQN GIQL LNGH APGA VPNL AGLQ QANR HHGL LG
Gene ID:118424
Uniprot:Q8N2K1
Cellular Location:Endoplasmic reticulum membrane, Single-pass type IV membrane protein
Calculated MW:24kDa/28kDa/30kDa
Observed MW:29kDa
Synonyms:UBE2J2, NCUBE-2, NCUBE2, PRO2121
Background:The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is located in the membrane of the endoplasmic reticulum. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined.
UniProt Protein Function:UBE2J2: Catalyzes the covalent attachment of ubiquitin to other proteins. Seems to function in the selective degradation of misfolded membrane proteins from the endoplasmic reticulum. Belongs to the ubiquitin-conjugating enzyme family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Ligase; Ubiquitin conjugating system; EC 6.3.2.19; Endoplasmic reticulum; Ubiquitin ligase; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p36.33

Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum; integral to membrane

Molecular Function:small conjugating protein ligase activity; ubiquitin protein ligase binding; ATP binding; ligase activity

Biological Process: ER-associated protein catabolic process; protein polyubiquitination; response to unfolded protein

NCBI Summary:The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is located in the membrane of the endoplasmic reticulum. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined. [provided by RefSeq, Jul 2008]
UniProt Code:Q8N2K1
NCBI GenInfo Identifier:251757431
NCBI Gene ID:118424
NCBI Accession:Q8N2K1.3
UniProt Secondary Accession:Q8N2K1,Q504T9, Q96N26, Q96T84, A8MYC7,
UniProt Related Accession:Q8N2K1
Molecular Weight:259
NCBI Full Name:Ubiquitin-conjugating enzyme E2 J2
NCBI Synonym Full Names:ubiquitin-conjugating enzyme E2, J2
NCBI Official Symbol:UBE2J2  
NCBI Official Synonym Symbols:NCUBE2; NCUBE-2; PRO2121  
NCBI Protein Information:ubiquitin-conjugating enzyme E2 J2; yeast UBC6 homolog; ubiquitin conjugating enzyme 6; ubiquitin conjugating enzyme E2, J2; non-canonical ubiquitin-conjugating enzyme 2; ubiquitin-conjugating enzyme E2, J2 (UBC6 homolog, yeast)
UniProt Protein Name:Ubiquitin-conjugating enzyme E2 J2
UniProt Synonym Protein Names:Non-canonical ubiquitin-conjugating enzyme 2; NCUBE-2
Protein Family:Ubiquitin-conjugating enzyme
UniProt Gene Name:UBE2J2  
UniProt Entry Name:UB2J2_HUMAN
Anti-UBE2J2 Antibody (CAB7316)Western blot analysis of extracts of various cell lines, using UBE2J2 antibody (CAB7316) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s.
Anti-UBE2J2 Antibody (CAB7316)Immunohistochemistry of paraffin-embedded rat liver using UBE2J2 antibody (CAB7316) at dilution of 1:100 (40x lens).
Anti-UBE2J2 Antibody (CAB7316)Immunofluorescence analysis of MCF-7 cells using UBE2J2 antibody (CAB7316). Blue: DAPI for nuclear staining.

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