Anti-Twist Antibody (CAB15596)[KO Validated]

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SKU:
CAB15596
Product type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Frequently bought together:

Description

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Antibody Name:Anti-Twist Antibody [KO Validated]
Antibody SKU:CAB15596
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Twist (NP_000465.1).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:SH-SY5Y, Mouse brain, 293T
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Twist (NP_000465.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MMQD VSSS PVSP ADDS LSNS EEEP DRQQ PPSG KRGG RKRR SSRR SAGG GAGP GGAA GGGV GGGD EPGS PAQG KRGK KSAG CGGG GGAG GGGG SSSG GGSP
Gene ID:7291
Uniprot:Q15672
Cellular Location:Nucleus
Calculated MW:20kDa
Observed MW:20kDa
Synonyms:TWIST1, ACS3, BPES2, BPES3, CRS, CRS1, CSO, SCS, TWIST, bHLHa38
Background:Basic helix-loop-helix (bHLH) transcription factors have been implicated in cell lineage determination and differentiation. The protein encoded by this gene is a bHLH transcription factor and shares similarity with another bHLH transcription factor, Dermo1. The strongest expression of this mRNA is in placental tissue; in adults, mesodermally derived tissues express this mRNA preferentially. Mutations in this gene have been found in patients with Saethre-Chotzen syndrome.
UniProt Protein Function:TWIST1: Acts as a transcriptional regulator. Inhibits myogenesis by sequestrating E proteins, inhibiting trans-activation by MEF2, and inhibiting DNA-binding by MYOD1 through physical interaction. This interaction probably involves the basic domains of both proteins. Also represses expression of proinflammatory cytokines such as TNFA and IL1B. Regulates cranial suture patterning and fusion. Activates transcription as a heterodimer with E proteins. Regulates gene expression differentially, depending on dimer composition. Homodimers induce expression of FGFR2 and POSTN while heterodimers repress FGFR2 and POSTN expression and induce THBS1 expression. Heterodimerization is also required for osteoblast differentiation. Efficient DNA binding requires dimerization with another bHLH protein. Homodimer or heterodimer with E proteins such as TCF3. ID1 binds preferentially to TCF3 but does not interact efficiently with TWIST1 so ID1 levels control the amount of TCF3 available to dimerize with TWIST1 and thus determine the type of dimer formed. Subset of mesodermal cells.
UniProt Protein Details:

Protein type:DNA-binding; Transcription regulation; Inhibitor; Cell development/differentiation

Chromosomal Location of Human Ortholog: 7p21.2

Cellular Component: nucleus

Molecular Function:protein domain specific binding; protein binding; protein homodimerization activity; protein heterodimerization activity; bHLH transcription factor binding; transcription factor binding

Biological Process: transcription from RNA polymerase II promoter; embryonic forelimb morphogenesis; negative regulation of skeletal muscle development; muscle development; rhythmic process; neuron migration; palate development; negative regulation of transcription from RNA polymerase II promoter; embryonic hindlimb morphogenesis; negative regulation of histone phosphorylation; negative regulation of histone acetylation; odontogenesis; negative regulation of DNA damage response, signal transduction by p53 class mediator; negative regulation of osteoblast differentiation; ossification; in utero embryonic development; negative regulation of transcription factor activity; outer ear morphogenesis; embryonic cranial skeleton morphogenesis; negative regulation of tumor necrosis factor production; positive regulation of tumor necrosis factor production; positive regulation of fatty acid beta-oxidation; osteoblast differentiation; positive regulation of angiogenesis; neural tube closure; positive regulation of transcription from RNA polymerase II promoter; embryonic digit morphogenesis; negative regulation of transcription, DNA-dependent; regulation of bone mineralization; positive regulation of epithelial cell proliferation; negative regulation of apoptosis; negative regulation of phosphoinositide 3-kinase cascade

Disease: Robinow-sorauf Syndrome; Craniosynostosis 1; Saethre-chotzen Syndrome

NCBI Summary:Basic helix-loop-helix (bHLH) transcription factors have been implicated in cell lineage determination and differentiation. The protein encoded by this gene is a bHLH transcription factor and shares similarity with another bHLH transcription factor, Dermo1. The strongest expression of this mRNA is in placental tissue; in adults, mesodermally derived tissues express this mRNA preferentially. Mutations in this gene have been found in patients with Saethre-Chotzen syndrome. [provided by RefSeq, Jul 2008]
UniProt Code:Q15672
NCBI GenInfo Identifier:2498009
NCBI Gene ID:7291
NCBI Accession:Q15672.1
UniProt Secondary Accession:Q15672,Q92487, Q99804, A4D128,
UniProt Related Accession:Q15672
Molecular Weight:Calculated: 20 kdaObserved: 34 kDa
NCBI Full Name:Twist-related protein 1
NCBI Synonym Full Names:twist family bHLH transcription factor 1
NCBI Official Symbol:TWIST1  
NCBI Official Synonym Symbols:CRS; CSO; SCS; ACS3; CRS1; BPES2; BPES3; TWIST; bHLHa38  
NCBI Protein Information:twist-related protein 1; H-twist; twist homolog 1; B-HLH DNA binding protein; TWIST homolog of drosophila; class A basic helix-loop-helix protein 38; twist basic helix-loop-helix transcription factor 1
UniProt Protein Name:Twist-related protein 1
UniProt Synonym Protein Names:Class A basic helix-loop-helix protein 38; bHLHa38; H-twist
Protein Family:Twist-related protein
UniProt Gene Name:TWIST1  
UniProt Entry Name:TWST1_HUMAN
Anti-Twist Antibody [KO Validated] (CAB15596)Western blot analysis of extracts of various cell lines, using Twist antibody (CAB15596) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-Twist Antibody [KO Validated] (CAB15596)Western blot analysis of extracts from normal (control) and Twist knockout (KO) 293T cells, using Twist antibody (CAB15596) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-Twist Antibody [KO Validated] (CAB15596)Immunohistochemistry of paraffin-embedded rat liver using Twist Rabbit pAb (CAB15596) at dilution of 1:200 (40x lens).
Anti-Twist Antibody [KO Validated] (CAB15596)Immunohistochemistry of paraffin-embedded human esophageal cancer using Twist Rabbit pAb (CAB15596) at dilution of 1:200 (40x lens).
Anti-Twist Antibody [KO Validated] (CAB15596)Immunohistochemistry of paraffin-embedded mouse lung using Twist Rabbit pAb (CAB15596) at dilution of 1:200 (40x lens).

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