Anti-TTK 항체 (CAB2500)

Antibody Name:	Anti-TTK Antibody
Antibody SKU:	CAB2500
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 517-856 of human TTK (NP_001160163.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 517-856 of human TTK (NP_001160163.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	ISVK GRIY SILK QIGS GGSS KVFQ VLNE KKQI YAIK YVNL EEAD NQTL DSYR NEIA YLNK LQQH SDKI IRLY DYEI TDQY IYMV MECG NIDL NSWL KKKK SIDP WERK SYWK NMLE AVHT IHQH GIVH SDLK PANF LIVD GMLK LIDF GIAN QMQP DTTS VVKD SQVG TVNY MPPE AIKD MSSS RENG KSKS KISP KSDV WSLG CILY YMTY GKTP FQQI INQI SKLH AIID PNHE IEFP DIPE KDLQ DVLK CCLK RDPK QRIS IPEL LAHP YVQI QTHP VNQM AKGT TEEM KYVL GQLV GLNS PNSI LKAA KTLY EHYS GGES HNSS SSKT FEKK RGKK
Gene ID:	7272
Uniprot:	P33981
Cellular Location:
Calculated MW:	96kDa/97kDa
Observed MW:	100KDa

Synonyms:

TTK, CT96, ESK, MPH1, MPS1, MPS1L1, PYT

Background:

This gene encodes a dual specificity protein kinase with the ability to phosphorylate tyrosine, serine and threonine. Associated with cell proliferation, this protein is essential for chromosome alignment at the centromere during mitosis and is required for centrosome duplication. It has been found to be a critical mitotic checkpoint protein for accurate segregation of chromosomes during mitosis. Tumorigenesis may occur when this protein fails to degrade and produces excess centrosomes resulting in aberrant mitotic spindles. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	TTK: a dual-specificity protein kinase orthologous to the yeast monopolar spindle (Mps1) kinase that is required for spindle pole duplication and the spindle checkpoint but not for centrosome duplication. Localized to centrosomes, kinetochores and nuclear pores. Its enzymatic activity is required for the recruitment of Mad1, Mad2, Bub1 and Bub3 to the kinetochore. Mps1 mRNA levels change little throughout the cell cycle, but its protein expression varies during the cell cycle. Overexpression arrests cells in metaphase by activating the checkpoint.
UniProt Protein Details:	Protein type:Protein kinase, Other; Kinase, protein; Protein kinase, dual-specificity (non-receptor); EC 2.7.12.1; Cancer Testis Antigen (CTA); Other group; TTK family Chromosomal Location of Human Ortholog: 6q14.1 Cellular Component: membrane; spindle Molecular Function:protein binding Biological Process: mitotic cell cycle spindle assembly checkpoint; mitotic spindle organization and biogenesis; positive regulation of cell proliferation; spindle organization and biogenesis
NCBI Summary:	This gene encodes a dual specificity protein kinase with the ability to phosphorylate tyrosine, serine and threonine. Associated with cell proliferation, this protein is essential for chromosome alignment at the centromere during mitosis and is required for centrosome duplication. It has been found to be a critical mitotic checkpoint protein for accurate segregation of chromosomes during mitosis. Tumorigenesis may occur when this protein fails to degrade and produces excess centrosomes resulting in aberrant mitotic spindles. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2009]
UniProt Code:	P33981
NCBI GenInfo Identifier:	160112977
NCBI Gene ID:	7272
NCBI Accession:	P33981.2
UniProt Secondary Accession:	P33981,Q15272, Q5TCS0, Q9BW51, Q9NTM0, A8K8U5, B2RDW2 E1P543,
UniProt Related Accession:	P33981
Molecular Weight:	96,944 Da
NCBI Full Name:	Dual specificity protein kinase TTK
NCBI Synonym Full Names:	TTK protein kinase
NCBI Official Symbol:	TTK
NCBI Official Synonym Symbols:	ESK; PYT; CT96; MPH1; MPS1; MPS1L1
NCBI Protein Information:	dual specificity protein kinase TTK
UniProt Protein Name:	Dual specificity protein kinase TTK
UniProt Synonym Protein Names:	Phosphotyrosine picked threonine-protein kinase; PYT
Protein Family:	Protein tramtrack
UniProt Gene Name:	TTK
UniProt Entry Name:	TTK_HUMAN

Western blot analysis of extracts of HeLa cells, using TTK antibody (CAB2500) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Antibodies

Phospho-TTK (T676) Antibody (PACO05263)

TTK Antibody (PACO05264)

TTK Antibody (PACO12977)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)