Anti-TTC3 Antibody (CAB15734)
- SKU:
- CAB15734
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Frequently bought together:
Description
| Antibody Name: | Anti-TTC3 Antibody |
| Antibody SKU: | CAB15734 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1400-1700 of human TTC3 (NP_003307.3). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-87MG, Mouse lung, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1400-1700 of human TTC3 (NP_003307.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | IASE TQIL EGSL GISV KSHC STGD AHTV LSES NRND EHCG NSNN KCEV IPES TSAV TNIP HVQM VAIQ VSWN IIHQ EVNT EPYN PFEE RQGE ISRI EKEH QVLQ DQLQ EVYE NYEQ IKLK GLEE TRDL EEKL KRHL EENK ISKT ELDW FLQD LERE IKKW QQEK KEIQ ERLK SLKK KIKK VSNA SEMY TQKN DGKE KEHE LHLD QSLE ISNT LTNE KMKI EEYI KKGK EDYE ESHQ RAVA AEVS VLEN WKES EVYK LQIM ESQA EAFL KKLG LISR DPAA YPDM ESDI RSWE LFLS NVTK E |
| Gene ID: | 7267 |
| Uniprot: | P53804 |
| Cellular Location: | Nucleus |
| Calculated MW: | 194kDa/203kDa/229kDa |
| Observed MW: | 280kDa |
| Synonyms: | TTC3, DCRR1, RNF105, TPRDIII |
| Background: |
| UniProt Protein Function: | TTC3: an E3 ubiquitin ligase that mediates the ubiquitination and subsequent degradation of phosphorylated Akt in the nucleus, acting to dampen Akt signaling after its activation. The human protein is phosphorylated on S378, a prerequisite for ubiquitin ligase activity. The kinase responsible for this phosphorylation may be Akt, suggestive of the existence of an auto-feedback loop that degrades Akts levels after it is activated. However the pattern of amino acids surrounding S378 only partially matches the canonical Akt motif, suggesting that kinase other than Akt may be responsible for the phosphorylation at S378. Catalyzes the formation of K48-polyubiquitin chains. May play a role in neuronal differentiation inhibition via its interaction with CRIK. TTC3 is one of the supernumerary genes in the Down?s syndrome critical region. Three alternatively spliced human isoforms of have been reported. |
| UniProt Protein Details: | Protein type:Ligase; Ubiquitin ligase; EC 6.3.2.-; Ubiquitin conjugating system; Cell development/differentiation; EC 6.3.2.19 Chromosomal Location of Human Ortholog: 21q22.2 Cellular Component: nucleoplasm; mitochondrion; vacuole; nucleus Molecular Function:protein binding; zinc ion binding; ubiquitin-protein ligase activity; ligase activity Biological Process: ubiquitin-dependent protein catabolic process; negative regulation of neuron differentiation |
| UniProt Code: | P53804 |
| NCBI GenInfo Identifier: | 49640011 |
| NCBI Gene ID: | 7267 |
| NCBI Accession: | NP_001001894.1 |
| UniProt Secondary Accession: | P53804,O60767, P78476, P78477, Q569I2, Q6P578, Q9UEK4 A8K7H7, B2RPA7, D3DSG9, D3DSH2, D3DSH3, |
| UniProt Related Accession: | P53804 |
| Molecular Weight: | 229,869 Da |
| NCBI Full Name: | E3 ubiquitin-protein ligase TTC3 |
| NCBI Synonym Full Names: | tetratricopeptide repeat domain 3 |
| NCBI Official Symbol: | TTC3 |
| NCBI Official Synonym Symbols: | DCRR1; RNF105; TPRDIII |
| NCBI Protein Information: | E3 ubiquitin-protein ligase TTC3; TPR repeat protein 3; TPR repeat protein D; RING finger protein 105; tetratricopeptide repeat protein 3 |
| UniProt Protein Name: | E3 ubiquitin-protein ligase TTC3 |
| UniProt Synonym Protein Names: | Protein DCRR1; RING finger protein 105; TPR repeat protein D; Tetratricopeptide repeat protein 3; TPR repeat protein 3 |
| Protein Family: | E3 ubiquitin-protein ligase |
| UniProt Gene Name: | TTC3 |
| UniProt Entry Name: | TTC3_HUMAN |
 | Western blot analysis of extracts of various cell lines, using TTC3 antibody (CAB15734) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 3min. |
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