Anti-TST Antibody (CAB10542)
- SKU:
- CAB10542
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-TST Antibody |
| Antibody SKU: | CAB10542 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IP |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-297 of human TST (NP_003303.2). |
| Application: | WB IP |
| Recommended Dilution: | WB 1:1000 - 1:2000 IP 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, DU145, MCF-7, 293T, Mouse liver, Mouse kidney, Mouse lung, Rat spinal cord |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-297 of human TST (NP_003303.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MVHQ VLYR ALVS TKWL AESI RTGK LGPG LRVL DASW YSPG TREA RKEY LERH VPGA SFFD IEEC RDTA SPYE MMLP SEAG FAEY VGRL GISN HTHV VVYD GEHL GSFY APRV WWMF RVFG HRTV SVLN GGFR NWLK EGHP VTSE PSRP EPAV FKAT LDRS LLKT YEQV LENL ESKR FQLV DSRS QGRF LGTE PEPD AVGL DSGH IRGA VNMP FMDF LTED GFEK GPEE LRAL FQTK KVDL SQPL IATC RKGV TACH VALA AYLC GKPD VAVY DGSW SEWF RRAP PESR VSQG KSEK A |
| Gene ID: | 7263 |
| Uniprot: | Q16762 |
| Cellular Location: | Mitochondrion matrix |
| Calculated MW: | 33kDa |
| Observed MW: | 36kDa |
| Synonyms: | TST, RDS |
| Background: | This is one of two neighboring genes encoding similar proteins that each contain two rhodanese domains. The encoded protein is localized to the mitochondria and catalyzes the conversion of thiosulfate and cyanide to thiocyanate and sulfite. In addition, the protein interacts with 5S ribosomal RNA and facilitates its import into the mitochondria. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | TST: Formation of iron-sulfur complexes, cyanide detoxification or modification of sulfur-containing enzymes. Other thiol compounds, besides cyanide, can act as sulfur ion acceptors. Also has weak mercaptopyruvate sulfurtransferase (MST) activity. Together with MRPL18, acts as a mitochondrial import factor for the cytosolic 5S rRNA. Only the nascent unfolded cytoplasmic form is able to bind to the 5S rRNA. |
| UniProt Protein Details: | Protein type:EC 2.8.1.1; Mitochondrial; Transferase Chromosomal Location of Human Ortholog: 22q12.3 Cellular Component: extracellular space; mitochondrion Molecular Function:5S rRNA binding Biological Process: cyanate catabolic process; epithelial cell differentiation; rRNA transport |
| NCBI Summary: | This is one of two neighboring genes encoding similar proteins that each contain two rhodanese domains. The encoded protein is localized to the mitochondria and catalyzes the conversion of thiosulfate and cyanide to thiocyanate and sulfite. In addition, the protein interacts with 5S ribosomal RNA and facilitates its import into the mitochondria. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2012] |
| UniProt Code: | Q16762 |
| NCBI GenInfo Identifier: | 3122965 |
| NCBI Gene ID: | 7263 |
| NCBI Accession: | Q16762.4 |
| UniProt Secondary Accession: | Q16762,Q6IB06, B3KRM1, |
| UniProt Related Accession: | Q16762 |
| Molecular Weight: | 33kDa |
| NCBI Full Name: | Thiosulfate sulfurtransferase |
| NCBI Synonym Full Names: | thiosulfate sulfurtransferase |
| NCBI Official Symbol: | TST |
| NCBI Official Synonym Symbols: | RDS |
| NCBI Protein Information: | thiosulfate sulfurtransferase |
| UniProt Protein Name: | Thiosulfate sulfurtransferase |
| UniProt Synonym Protein Names: | Rhodanese |
| Protein Family: | Toxic shock syndrome toxin |
| UniProt Gene Name: | TST |
 | Western blot analysis of extracts of various cell lines, using TST antibody (CAB10542) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
 | Immunoprecipitation analysis of 200ug extracts of A-549 cells, using 3 ug TST antibody (CAB10542). Western blot was performed from the immunoprecipitate using TST antibody (CAB10542) at a dilition of 1:1000. |
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