Anti-TRUB2 Antibody (CAB15818)
- SKU:
- CAB15818
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Frequently bought together:
Description
| Antibody Name: | Anti-TRUB2 Antibody |
| Antibody SKU: | CAB15818 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 122-331 of human TRUB2 (NP_056494.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | K-562, HeLa |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 122-331 of human TRUB2 (NP_056494.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | AHLT KDYT VRGL LGKA TDDF REDG RLVE KTTY DHVT REKL DRIL AVIQ GSHQ KALV MYSN LDLK TQEA YEMA VRGL IRPM NKSP MLIT GIRC LYFA PPEF LLEV QCMH ETQK ELRK LVHE IGLE LKTT AVCT QVRR TRDG FFTL DSAL LRTQ WDLT NIQD AIRA ATPQ VAAE LEKS LSPG LDTK QLPS PGWS WDSQ GPSS TLGL ERGA GQ |
| Gene ID: | 26995 |
| Uniprot: | O95900 |
| Cellular Location: | |
| Calculated MW: | 30kDa/36kDa |
| Observed MW: | 37kDa |
| Synonyms: | TRUB2, CLONE24922 |
| Background: | Pseudouridine is an abundant component of rRNAs and tRNAs and is enzymatically generated by isomerization of uridine by pseudouridine synthase (Zucchini et al., 2003 [PubMed 12736709]). |
| UniProt Protein Function: | TRUB2: May be responsible for synthesis of pseudouridine from uracil in transfer RNAs. Belongs to the pseudouridine synthase TruB family. |
| UniProt Protein Details: | Protein type:EC 5.4.99.-; RNA processing; RNA-binding; Isomerase Chromosomal Location of Human Ortholog: 9q34.11 Cellular Component: cytoplasm Molecular Function:pseudouridine synthase activity Biological Process: RNA processing; pseudouridine synthesis; tRNA processing |
| NCBI Summary: | Pseudouridine is an abundant component of rRNAs and tRNAs and is enzymatically generated by isomerization of uridine by pseudouridine synthase (Zucchini et al., 2003 [PubMed 12736709]).[supplied by OMIM, Mar 2008] |
| UniProt Code: | O95900 |
| NCBI GenInfo Identifier: | 74739707 |
| NCBI Gene ID: | 26995 |
| NCBI Accession: | O95900.1 |
| UniProt Secondary Accession: | O95900,B7Z7G5, |
| UniProt Related Accession: | O95900 |
| Molecular Weight: | 331 |
| NCBI Full Name: | Probable tRNA pseudouridine synthase 2 |
| NCBI Synonym Full Names: | TruB pseudouridine (psi) synthase family member 2 |
| NCBI Official Symbol: | TRUB2 |
| NCBI Official Synonym Symbols: | CLONE24922 |
| NCBI Protein Information: | probable tRNA pseudouridine synthase 2; TruB pseudouridine (psi) synthase homolog 2 |
| UniProt Protein Name: | Probable tRNA pseudouridine synthase 2 |
| Protein Family: | Probable tRNA pseudouridine synthase |
| UniProt Gene Name: | TRUB2 |
| UniProt Entry Name: | TRUB2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using TRUB2 antibody (CAB15818) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s. |
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