Anti-TRIM32 항체 (CAB7079)

Antibody Name:	Anti-TRIM32 Antibody
Antibody SKU:	CAB7079
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human TRIM32 (NP_036342.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, Mouse testis, mouse brain, rat testis, rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human TRIM32 (NP_036342.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAAA AASH LNLD ALRE VLEC PICM ESFT EEQL RPKL LHCG HTIC RQCL EKLL ASSI NGVR CPFC SKIT RITS LTQL TDNL TVLK IIDT AGLS EAVG LLMC RSCG RRLP RQFC RSCG LVLC EPCR EADH QPPG HCTL PVKE AAEE RRRD FGEK LTRL RELM GELQ RRKA ALEG VSKD LQAR YKAV LQEY GHEE RRVQ DELA RSRK FFTG SLAE VEKS NSQV VEEQ SYLL NIAE VQAV SRCD YFLA KIKQ ADVA LLEE TADE EEPE LTAS LPRE LTLQ DVEL
Gene ID:	22954
Uniprot:	Q13049
Cellular Location:	Cytoplasm
Calculated MW:	71kDa
Observed MW:	75kDa

Synonyms:

TRIM32, BBS11, HT2A, LGMD2H, TATIP

Background:

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic bodies. The protein has also been localized to the nucleus, where it interacts with the activation domain of the HIV-1 Tat protein. The Tat protein activates transcription of HIV-1 genes.

UniProt Protein Function:	TRIM32: Has an E3 ubiquitin ligase activity. Ubiquitinates DTNBP1 (dysbindin) and promotes its degradation. May play a significant role in mediating the biological activity of the HIV-1 Tat protein in vivo. Binds specifically to the activation domain of HIV-1 Tat and can also interact with the HIV-2 and EIAV Tat proteins in vivo. Defects in TRIM32 are the cause of limb-girdle muscular dystrophy type 2H (LGMD2H); also known as muscular dystrophy Hutterite type. LGMD2H is an autosomal recessive degenerative myopathy characterized by pelvic girdle, shoulder girdle and quadriceps muscle weakness. Clinical phenotype and severity are highly variable. Disease progression is slow and most patients remain ambulatory into the sixth decade of life. Defects in TRIM32 are the cause of Bardet-Biedl syndrome type 11 (BBS11). Bardet-Biedl syndrome (BBS) is a genetically heterogeneous, autosomal recessive disorder characterized by usually severe pigmentary retinopathy, early onset obesity, polydactyly, hypogenitalism, renal malformation and mental retardation. Secondary features include diabetes mellitus, hypertension and congenital heart disease. A relatively high incidence of BBS is found in the mixed Arab populations of Kuwait and in Bedouin tribes throughout the Middle East, most likely due to the high rate of consaguinity in these populations and a founder effect. Belongs to the TRIM/RBCC family.
UniProt Protein Details:	Protein type:Ligase; EC 6.3.2.19; Ubiquitin conjugating system; EC 6.3.2.-; Ubiquitin ligase Chromosomal Location of Human Ortholog: 9q33.1 Cellular Component: cytoplasm; striated muscle thick filament; cytosol; nucleus Molecular Function:protein binding; ubiquitin binding; protein self-association; myosin binding; zinc ion binding; Tat protein binding; RNA binding; translation initiation factor binding; transcription coactivator activity; ubiquitin-protein ligase activity; ligase activity Biological Process: fat cell differentiation; regulation of interferon type I production; positive regulation of neurogenesis; protein polyubiquitination; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of proteolysis; protein ubiquitination during ubiquitin-dependent protein catabolic process; protein ubiquitination; positive regulation of cell cycle; positive regulation of cell growth; activation of NF-kappaB transcription factor; negative regulation of viral transcription; negative regulation of fibroblast proliferation; positive regulation of protein catabolic process; positive regulation of interferon type I production; innate immune response; positive regulation of transcription factor activity; positive regulation of neuron differentiation; response to UV; positive regulation of cell migration Disease: Muscular Dystrophy, Limb-girdle, Type 2h; Bardet-biedl Syndrome 11
NCBI Summary:	The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic bodies. The protein has also been localized to the nucleus, where it interacts with the activation domain of the HIV-1 Tat protein. The Tat protein activates transcription of HIV-1 genes. [provided by RefSeq, Jul 2008]
UniProt Code:	Q13049
NCBI GenInfo Identifier:	20178303
NCBI Gene ID:	22954
NCBI Accession:	Q13049.2
UniProt Secondary Accession:	Q13049,Q9NQP8,
UniProt Related Accession:	Q13049
Molecular Weight:	653
NCBI Full Name:	E3 ubiquitin-protein ligase TRIM32
NCBI Synonym Full Names:	tripartite motif containing 32
NCBI Official Symbol:	TRIM32
NCBI Official Synonym Symbols:	HT2A; BBS11; TATIP; LGMD2H
NCBI Protein Information:	E3 ubiquitin-protein ligase TRIM32; zinc finger protein HT2A; zinc-finger protein HT2A; 72 kDa Tat-interacting protein; TAT-interactive protein, 72-KD; tripartite motif-containing 32; tripartite motif-containing protein 32
UniProt Protein Name:	E3 ubiquitin-protein ligase TRIM32
UniProt Synonym Protein Names:	72 kDa Tat-interacting protein; Tripartite motif-containing protein 32; Zinc finger protein HT2A
Protein Family:	E3 ubiquitin-protein ligase
UniProt Gene Name:	TRIM32
UniProt Entry Name:	TRI32_HUMAN

	Western blot analysis of extracts of various cell lines, using TRIM32 antibody (CAB7079) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Western blot analysis of extracts of HeLa cells, using TRIM32 antibody (CAB7079) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.

Antibodies

TRIM32 Antibody (PACO12914)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)