Anti-TRIM25 Antibody (CAB19887)[KO Validated]

(No reviews yet) Write a Review
SKU:
CAB19887
Product type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Immunology
Frequently bought together:

Description

system_update_alt데이터시트system_update_alt안전 데이터시트
Antibody Name:Anti-TRIM25 Antibody [KO Validated]
Antibody SKU:CAB19887
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IP
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant protein of human TRIM25.
Application:WB IHC IP
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100 IP 1:50 - 1:100
Reactivity:Human, Mouse
Positive Samples:A-549
Immunogen:Recombinant protein of human TRIM25.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:7706
Uniprot:Q14258
Cellular Location:Cytoplasm
Calculated MW:70kDa
Observed MW:71KDa
Anti-TRIM25 Antibody [KO Validated] (CAB19887)Western blot analysis of extracts from normal (control) and TRIM25 knockout (KO) A-549 cells, using TRIM25 antibody (CAB19887) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10S.
Synonyms:TRIM25, EFP, RNF147, Z147, ZNF147
Background:The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the cytoplasm. The presence of potential DNA-binding and dimerization-transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene.
UniProt Protein Function:TRIM25: a ubiquitous protein that mediates estrogen action in various target organs. Contains one RING-type zinc finger and one SPRY domain.
UniProt Protein Details:

Protein type:EC 6.3.2.19; Ubiquitin conjugating system; Transcription factor; Ubiquitin ligase; EC 6.3.2.n3; Ligase; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 17q23.2

Cellular Component: nucleoplasm; cytoplasm; cytosol

Molecular Function:protein binding; acid-amino acid ligase activity; zinc ion binding; transcription factor activity

Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; viral reproduction; negative regulation of virion penetration into host cell; cytokine and chemokine mediated signaling pathway; protein ubiquitination; regulation of virion penetration into host cell; activation of NF-kappaB transcription factor; response to vitamin D; response to estrogen stimulus; innate immune response; positive regulation of transcription factor activity; defense response to virus; negative regulation of interferon type I production

NCBI Summary:The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the cytoplasm. The presence of potential DNA-binding and dimerization-transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene. [provided by RefSeq, Jul 2008]
UniProt Code:Q14258
NCBI GenInfo Identifier:313104033
NCBI Gene ID:7706
NCBI Accession:Q14258.2
UniProt Related Accession:Q14258
Molecular Weight:630
NCBI Full Name:E3 ubiquitin/ISG15 ligase TRIM25
NCBI Synonym Full Names:tripartite motif containing 25
NCBI Official Symbol:TRIM25  
NCBI Official Synonym Symbols:EFP; Z147; RNF147; ZNF147  
NCBI Protein Information:E3 ubiquitin/ISG15 ligase TRIM25; RING finger protein 147; zinc finger protein-147; tripartite motif-containing 25; tripartite motif protein TRIM25; estrogen-responsive finger protein; tripartite motif-containing protein 25; ubiquitin/ISG15-conjugating enzyme TRIM25; zinc finger protein 147 (estrogen-responsive finger protein)
UniProt Protein Name:E3 ubiquitin/ISG15 ligase TRIM25
UniProt Synonym Protein Names:Estrogen-responsive finger protein; RING finger protein 147; Tripartite motif-containing protein 25; Ubiquitin/ISG15-conjugating enzyme TRIM25; Zinc finger protein 147
Protein Family:E3 ubiquitin/ISG15 ligase
UniProt Gene Name:TRIM25  
UniProt Entry Name:TRI25_HUMAN

0 Reviews

View AllClose

Related Products