Anti-TRIM24 Antibody (CAB10546)

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SKU:
CAB10546
Product type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
Frequently bought together:

Description

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Antibody Name:Anti-TRIM24 Antibody
Antibody SKU:CAB10546
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 600-820 of human TRIM24 (NP_056989.2).
Application:WB
Recommended Dilution:WB 1:1000 - 1:2000
Reactivity:Human
Positive Samples:293T, HeLa
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 600-820 of human TRIM24 (NP_056989.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:DGKA FGSP MIDL SSPV GGSY NLPS LPDI DCSS TIML DNIV RKDT NIDH GQPR PPSN RTVQ SPNS SVPS PGLA GPVT MTSV HPPI RSPS ASSV GSRG SSGS SSKP AGAD STHK VPVV MLEP IRIK QENS GPPE NYDF PVVI VKQE SDEE SRPQ NANY PRSI LTSL LLNS SQSS TSEE TVLR SDAP DSTG DQPG LHQD NSSN GKSE WLDP SQKS PLHV GETR K
Gene ID:8805
Uniprot:O15164
Cellular Location:Cytoplasm, Nucleus
Calculated MW:113kDa/116kDa
Observed MW:117kDa
Synonyms:TRIM24, PTC6, RNF82, TF1A, TIF1, TIF1A, TIF1ALPHA, hTIF1
Background:The protein encoded by this gene mediates transcriptional control by interaction with the activation function 2 (AF2) region of several nuclear receptors, including the estrogen, retinoic acid, and vitamin D3 receptors. The protein localizes to nuclear bodies and is thought to associate with chromatin and heterochromatin-associated factors. The protein is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains - a RING, a B-box type 1 and a B-box type 2 - and a coiled-coil region. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene.
UniProt Protein Function:TRIM24: an atypical protein kinase that functions as a transcriptional coactivator. Originally identified as a mammalian protein that enhances the AF-2 activity of the retinoid X receptor (RXR) in a yeast genetic screen. Binds to nuclear receptors in an agonist and AF2-activating domain (AF2-AD) dependent manner. Binding to the agonist-nuclear receptor complex induces its own hyperphosphorylation as well as the phosphorylation of TFIIEa, TAFII28, and TAFII55. Belongs to a family of nuclear proteins that includes TIF1b and TIF1g. TIF1 proteins are characterized by an N-terminal region that contains a RING finger-B boxes-coiled coil (RBCC) motif, a poorly conserved central region, a C-terminal region that contains a PHD finger, and a bromodomain. Interacts with the heterochromatin-associated proteins HP1a, MOD1 (HP1b), and MOD2 (HP1g) and with the transcriptional repression domain KRAB, which is present in many Kruppel-type (C2H2) zinc finger proteins. May act as a repressor through the formation of transcriptionally inactive heterochromatin. Two splice variant isoforms have been described.
UniProt Protein Details:

Protein type:ATYPICAL group; EC 6.3.2.-; Kinase, protein; Nuclear receptor co-regulator; Protein kinase, atypical; TIF1 family; Transcription, coactivator/corepressor; Tumor suppressor; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 7q33-q34

Cellular Component: cytosol; nucleoplasm; nucleus

Molecular Function:chromatin binding; estrogen response element binding; methylated histone residue binding; p53 binding; protein binding; protein-tyrosine kinase activity; receptor binding; transcription coactivator activity; ubiquitin-protein ligase activity; zinc ion binding

Biological Process: protein catabolic process; protein ubiquitination; regulation of apoptosis; regulation of protein stability; transcription from RNA polymerase II promoter

Disease: Thyroid Carcinoma, Papillary

NCBI Summary:The protein encoded by this gene mediates transcriptional control by interaction with the activation function 2 (AF2) region of several nuclear receptors, including the estrogen, retinoic acid, and vitamin D3 receptors. The protein localizes to nuclear bodies and is thought to associate with chromatin and heterochromatin-associated factors. The protein is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains - a RING, a B-box type 1 and a B-box type 2 - and a coiled-coil region. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:O15164
NCBI GenInfo Identifier:12746552
NCBI Gene ID:8805
NCBI Accession:O15164.3
UniProt Secondary Accession:O15164,O95854, A4D1R7, A4D1R8,
UniProt Related Accession:O15164
Molecular Weight:117kDa
NCBI Full Name:Transcription intermediary factor 1-alpha
NCBI Synonym Full Names:tripartite motif containing 24
NCBI Official Symbol:TRIM24  
NCBI Official Synonym Symbols:PTC6; TF1A; TIF1; RNF82; TIF1A; hTIF1; TIF1ALPHA  
NCBI Protein Information:transcription intermediary factor 1-alpha
UniProt Protein Name:Transcription intermediary factor 1-alpha
UniProt Synonym Protein Names:E3 ubiquitin-protein ligase TRIM24; RING finger protein 82; RING-type E3 ubiquitin transferase TIF1-alphaCurated; Tripartite motif-containing protein 24
Protein Family:Transcription intermediary factor
UniProt Gene Name:TRIM24  
Anti-TRIM24 Antibody (CAB10546)Western blot analysis of extracts of various cell lines, using TRIM24 antibody (CAB10546) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.

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