Anti-TRAPPC6A Antibody (CAB16143)

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SKU:
CAB16143
Product type:
Antibody
Reactivity:
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Frequently bought together:

Description

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Antibody Name:Anti-TRAPPC6A Antibody
Antibody SKU:CAB16143
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-173 of human TRAPPC6A (NP_077013.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Mouse, Rat
Positive Samples:Mouse kidney, Rat kidney, Rat liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-173 of human TRAPPC6A (NP_077013.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MADT VLFE FLHT EMVA ELWA HDPD PGPG VSAG LRGE EAGA TKGQ KMSL SVLE GMGF RVGQ ALGE RLPR ETLA FREE LDVL KFLC KDLW VAVF QKQM DSLR TNHQ GTYV LQDN SFPL LLPM ASGL QYLE EAPK FLAF TCGL LRGA LYTL GIES VVTA SVAA LPVC KFQV VIPK S
Gene ID:79090
Uniprot:O75865
Cellular Location:Endoplasmic reticulum, Golgi apparatus, cis-Golgi network
Calculated MW:11kDa/12kDa/17kDa/18kDa
Observed MW:18kDa
Synonyms:TRAPPC6A, TRS33
Background:This gene encodes a component of the trafficking protein particle complex, which tethers transport vesicles to the cis-Golgi membrane. Loss of expression of the related gene in mouse affects coat and eye pigmentation, suggesting that the encoded protein may be involved in melanosome biogenesis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
UniProt Protein Function:TRAPPC6A: May play a role in vesicular transport during the biogenesis of melanosomes. Belongs to the TRAPP small subunits family. BET3 subfamily. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Chromosomal Location of Human Ortholog: 19q13.32

Cellular Component: cytosol

Molecular Function:protein binding

Biological Process: COPII coating of Golgi vesicle

NCBI Summary:This gene encodes a component of the trafficking protein particle complex, which tethers transport vesicles to the cis-Golgi membrane. Loss of expression of the related gene in mouse affects coat and eye pigmentation, suggesting that the encoded protein may be involved in melanosome biogenesis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2012]
UniProt Code:O75865
NCBI GenInfo Identifier:109895221
NCBI Gene ID:79090
NCBI Accession:O75865.2
UniProt Secondary Accession:O75865,Q9BQ45, Q9P092, K7ERB1, K7ERQ4,
UniProt Related Accession:O75865
Molecular Weight:12,976 Da
NCBI Full Name:Trafficking protein particle complex subunit 6A
NCBI Synonym Full Names:trafficking protein particle complex 6A
NCBI Official Symbol:TRAPPC6A  
NCBI Official Synonym Symbols:TRS33  
NCBI Protein Information:trafficking protein particle complex subunit 6A
UniProt Protein Name:Trafficking protein particle complex subunit 6A
Protein Family:Trafficking protein particle complex
UniProt Gene Name:TRAPPC6A  
UniProt Entry Name:TPC6A_HUMAN
Anti-TRAPPC6A Antibody (CAB16143)Western blot analysis of extracts of various cell lines, using TRAPPC6A antibody (CAB16143) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-TRAPPC6A Antibody (CAB16143)Immunofluorescence analysis of L929 cells using TRAPPC6A antibody (CAB16143) at dilution of 1:100. Blue: DAPI for nuclear staining.

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