Anti-Transportin-1 Antibody (CAB9435)
- SKU:
- CAB9435
- Product type:
- Antibody
- Application:
- WB
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Immunology
Frequently bought together:
Description
| Antibody Name: | Anti-Transportin-1 Antibody |
| Antibody SKU: | CAB9435 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 800 to the C-terminus of human TNPO1 (NP_002261.3). |
| Application: | WB |
| Recommended Dilution: | WB 1:200 - 1:1000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | LO2, U-87MG, DU145, HeLa, Mouse kidney, Rat kidney |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 800 to the C-terminus of human TNPO1 (NP_002261.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | QFIR PWCT SLRN IRDN EEKD SAFR GICT MISV NPSG VIQD FIFF CDAV ASWI NPKD DLRD MFCK ILHG FKNQ VGDE NWRR FSDQ FPLP LKER LAAF YGV |
| Gene ID: | 3842 |
| Uniprot: | Q92973 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 96kDa/101kDa/102kDa |
| Observed MW: | 85-102kDa |
| Synonyms: | TNPO1, IPO2, KPNB2, MIP, MIP1, TRN |
| Background: | This gene encodes the beta subunit of the karyopherin receptor complex which interacts with nuclear localization signals to target nuclear proteins to the nucleus. The karyopherin receptor complex is a heterodimer of an alpha subunit which recognizes the nuclear localization signal and a beta subunit which docks the complex at nucleoporins. Alternate splicing of this gene results in two transcript variants encoding different proteins. |
| UniProt Protein Function: | KPNB2: Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran- dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Involved in nuclear import of M9- containing proteins. In vitro, binds directly to the M9 region of the heterogeneous nuclear ribonucleoproteins (hnRNP), A1 and A2 and mediates their nuclear import. Appears also to be involved in hnRNP A1/A2 nuclear export. Mediates the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones, and SRP19. In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev. Belongs to the importin beta family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Karyopherin; RNA-binding; Nuclear import Chromosomal Location of Human Ortholog: 5q13.2 Cellular Component: cytoplasm; cytosol; nuclear membrane; nucleus Molecular Function:nuclear localization sequence binding; protein binding; protein transporter activity Biological Process: NLS-bearing substrate import into nucleus; protein import into nucleus, docking; protein import into nucleus, translocation; regulation of mRNA stability; ribosomal protein import into nucleus |
| NCBI Summary: | This gene encodes the beta subunit of the karyopherin receptor complex which interacts with nuclear localization signals to target nuclear proteins to the nucleus. The karyopherin receptor complex is a heterodimer of an alpha subunit which recognizes the nuclear localization signal and a beta subunit which docks the complex at nucleoporins. Alternate splicing of this gene results in two transcript variants encoding different proteins. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q92973 |
| NCBI GenInfo Identifier: | 259016171 |
| NCBI Gene ID: | 3842 |
| NCBI Accession: | Q92973.2 |
| UniProt Secondary Accession: | Q92973,Q92957, Q92975, B4DVC6, |
| UniProt Related Accession: | Q92973 |
| Molecular Weight: | 96,901 Da |
| NCBI Full Name: | Transportin-1 |
| NCBI Synonym Full Names: | transportin 1 |
| NCBI Official Symbol: | TNPO1 |
| NCBI Official Synonym Symbols: | MIP; TRN; IPO2; MIP1; KPNB2 |
| NCBI Protein Information: | transportin-1 |
| UniProt Protein Name: | Transportin-1 |
| UniProt Synonym Protein Names: | Importin beta-2; Karyopherin beta-2; M9 region interaction protein; MIP |
| UniProt Gene Name: | TNPO1 |
| UniProt Entry Name: | TNPO1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using TNPO1 antibody (CAB9435) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 1s. |
Related Products
Anti-Transportin 3 (TNPO3) Antibody (CAB19774)
Mouse Transportin 1 / TNPO1 ELISA Kit
Human Transportin 1 / TNPO1 ELISA Kit
Anti-Nesprin 1 Antibody (CAB19781)
