Anti-TRAF2 Antibody (CAB0962)[KO Validated]

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SKU:
CAB0962
Product type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death
Frequently bought together:

Description

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Antibody Name:Anti-TRAF2 Antibody [KO Validated]
Antibody SKU:CAB0962
Antibody Size:20uL, 50uL, 100uL
Application:WB IF IP
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human TRAF2 (NP_066961.2).
Application:WB IF IP
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:100 IP 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, 293T, THP-1, PC-12, Ramos, Jurkat, NIH/3T3
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human TRAF2 (NP_066961.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MAAA SVTP PGSL ELLQ PGFS KTLL GTKL EAKY LCSA CRNV LRRP FQAQ CGHR YCSF CLAS ILSS GPQN CAAC VHEG IYEE GISI LESS SAFP DNAA RREV ESLP AVCP SDGC TWKG TLKE YESC HEGR CPLM LTEC PACK GLVR LGEK ERHL EHEC PERS LSCR HCRA PCCG ADVK AHHE VCPK FPLT CDGC GKKK IPRE KFQD HVKT CGKC RVPC RFHA IGCL ETVE GEKQ QEHE VQWL REHL AMLL SSVL EAKP LLGD QSHA GSEL LQRC ESLE KKTA TFEN IVCV LNRE VERV AMTA
Gene ID:7186
Uniprot:Q12933
Cellular Location:Cytoplasm
Calculated MW:53kDa/54kDa/55kDa/61kDa
Observed MW:54kDa
Synonyms:MGC:45012, TRAP, TRAP3, TRAF2
Background:The protein encoded by this gene is a member of the TNF receptor associated factor (TRAF) protein family. TRAF proteins associate with, and mediate the signal transduction from members of the TNF receptor superfamily. This protein directly interacts with TNF receptors, and forms a heterodimeric complex with TRAF1. This protein is required for TNF-alpha-mediated activation of MAPK8/JNK and NF-kappaB. The protein complex formed by this protein and TRAF1 interacts with the inhibitor-of-apoptosis proteins (IAPs), and functions as a mediator of the anti-apoptotic signals from TNF receptors. The interaction of this protein with TRADD, a TNF receptor associated apoptotic signal transducer, ensures the recruitment of IAPs for the direct inhibition of caspase activation. BIRC2/c-IAP1, an apoptosis inhibitor possessing ubiquitin ligase activity, can unbiquitinate and induce the degradation of this protein, and thus potentiate TNF-induced apoptosis. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of only one transcript has been determined.
UniProt Protein Function:TRAF2: Regulates activation of NF-kappa-B and JNK and plays a central role in the regulation of cell survival and apoptosis. Required for normal antibody isotype switching from IgM to IgG. Has E3 ubiquitin-protein ligase activity and promotes 'Lys-63'- linked ubiquitination of target proteins, such as BIRC3, RIPK1 and TICAM1. Is an essential constituent of several E3 ubiquitin- protein ligase complexes, where it promotes the ubiquitination of target proteins by bringing them into contact with other E3 ubiquitin ligases. Regulates BIRC2 and BIRC3 protein levels by inhibiting their autoubiquitination and subsequent degradation; this does not depend on the TRAF2 RING-type zinc finger domain. Homotrimer, and heterotrimer with TRAF1 and TRAF3 (via TRAF domain). The domain containing the RING-type and the first TRAF-type zinc finger can also form homodimers (in vitro). Interacts with TNFRSF1B/TNFR2, TNFRSF4, TNFRSF5/CD40, CD27/TNFRSF7, TNFRSF8/CD30, TNFRSF9/CD137, TNFRSF11A/RANK, TNFRSF13B/TACI, TNFRSF14, TNFRSF16/NGFR, TNFRSF17/BCMA, TNFRSF18/AITR, TNFRSF19/TROY, TNFRSF19L/RELT, XEDAR, EDAR, Epstein-Barr virus BNFL1/LMP-1 and IL15RA. Interacts with CDK9, CSK, MAP3K1, MAP3K5, MAP3K11, MAP3K14, MAP4K2, RIPK1, RIPK2, TNIK, TBK1, SPHK1, TRADD, TRAFD1, TRAIP, TANK/ITRAF, TNFAIP3, TDP2, MAVS/IPS1, TICAM1 and TRPC4AP. Interacts with CASP8AP2, NFATC2IP, PEG3 and HIVEP3. Interacts with BIRC2 and BIRC3 N- terminus; a single BIRC2 or BIRC3 molecule interacts with a heterotrimer formed by TRAF1 and TRAF2, or a TRAF2 homotrimer. Identified in a complex composed of TRAF2, TRAF3, BIRC2 and BIRC3. Interaction with BIRC2 and/or BIRC3 is essential for degradation of NFKBIA and activation of NF-kappa-B. Interacts with CYLD, USP48, DAB2IP, IKKA and IKKB. Identified in a complex with TNFRSF1A, RIPK1 and IKKB. Interacts (via 'Lys-63'-linked polyubiquitin chains) with TAB2 and TAB3. Interacts with ERN1 and TAOK3. Interaction with TAOK3 is facilitated under ER stress conditions, such as treatment with tunicamycin, and may promote TRAF2 phosphorylation. Has very low E3 ubiquitin ligase activity in the absence of sphingosine-1-phosphate. E3 ubiquitin ligase activity is strongly activated by cytoplasmic sphingosine-1- phosphate. Belongs to the TNF receptor-associated factor family. A subfamily. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Ligase; Ubiquitin conjugating system; Autophagy; Ubiquitin ligase; EC 6.3.2.-

Chromosomal Location of Human Ortholog: 9q34

Cellular Component: internal side of plasma membrane; cytoplasm; vesicle membrane; cell cortex; cytosol; ubiquitin ligase complex; lipid raft

Molecular Function:identical protein binding; sphingolipid binding; zinc ion binding; CD40 receptor binding; ubiquitin-protein ligase activity; mitogen-activated protein kinase kinase kinase binding; tumor necrosis factor receptor binding; protein phosphatase binding; protein binding; signal transducer activity; enzyme binding; ubiquitin protein ligase binding; thioesterase binding; protein complex binding; ligase activity

Biological Process: caspase activation; positive regulation of JNK activity; protein autoubiquitination; protein heterooligomerization; apoptosis; activation of NF-kappaB-inducing kinase; positive regulation of T cell cytokine production; signal transduction; activation of NF-kappaB transcription factor; regulation of apoptosis; cellular protein complex assembly; tumor necrosis factor-mediated signaling pathway; positive regulation of interleukin-2 production; innate immune response; protein catabolic process; protein complex assembly; positive regulation of transcription factor activity; positive regulation of T cell activation; regulation of immunoglobulin secretion

UniProt Code:Q12933
NCBI GenInfo Identifier:22027614
NCBI Gene ID:
NCBI Accession:NP_663770
Molecular Weight:55.729
NCBI Full Name:
UniProt Protein Name:TNF receptor-associated factor 2
UniProt Synonym Protein Names:E3 ubiquitin-protein ligase TRAF2; Tumor necrosis factor type 2 receptor-associated protein 3
Protein Family:TNF receptor-associated factor
UniProt Gene Name:TRAF2  
UniProt Entry Name:TRAF2_HUMAN
Anti-TRAF2 Antibody [KO Validated] (CAB0962)Western blot analysis of extracts of various cell lines, using TRAF2 antibody (CAB0962) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Anti-TRAF2 Antibody [KO Validated] (CAB0962)Western blot analysis of extracts from normal (control) and TRAF2 knockout (KO) 293T cells, using TRAF2 antibody (CAB0962) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 6s.
Anti-TRAF2 Antibody [KO Validated] (CAB0962)Immunofluorescence analysis of HeLa cells using TRAF2 antibody (CAB0962). Blue: DAPI for nuclear staining.

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