Anti-TOM20 Antibody (CAB6774)
- SKU:
- CAB6774
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-TOM20 Antibody |
| Antibody SKU: | CAB6774 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF IP |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 20-145 of human TOM20 (NP_055580.1). |
| Application: | WB IHC IF IP |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:20 - 1:50 IP 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | THP-1, HL-60, HepG2, MCF7, Mouse kideny, Mouse brain, Rat thymus |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 20-145 of human TOM20 (NP_055580.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | YCIY FDRK RRSD PNFK NRLR ERRK KQKL AKER AGLS KLPD LKDA EAVQ KFFL EEIQ LGEE LLAQ GEYE KGVD HLTN AIAV CGQP QQLL QVLQ QTLP PPVF QMLL TKLP TISQ RIVS AQSL AEDD VE |
| Gene ID: | 9804 |
| Uniprot: | Q15388 |
| Cellular Location: | Mitochondrion outer membrane, Single-pass membrane protein |
| Calculated MW: | 16kDa |
| Observed MW: | 16kDa |
| Synonyms: | TOMM20, MAS20, MOM19, TOM20 |
| Background: |
| UniProt Protein Function: | TOM20: Central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore. Belongs to the Tom20 family. |
| UniProt Protein Details: | Protein type:Mitochondrial; Membrane protein, integral Chromosomal Location of Human Ortholog: 1q42 Cellular Component: mitochondrial outer membrane translocase complex; mitochondrion; integral to membrane Molecular Function:protein binding; P-P-bond-hydrolysis-driven protein transmembrane transporter activity; unfolded protein binding Biological Process: cellular protein metabolic process; protein targeting to mitochondrion |
| UniProt Code: | Q15388 |
| NCBI GenInfo Identifier: | 2498697 |
| NCBI Gene ID: | 9804 |
| NCBI Accession: | Q15388.1 |
| UniProt Secondary Accession: | Q15388,Q498B3, Q6IBT4, A8K195, |
| UniProt Related Accession: | Q15388,AAB35420 |
| Molecular Weight: | 145 |
| NCBI Full Name: | Mitochondrial import receptor subunit TOM20 homolog |
| NCBI Synonym Full Names: | translocase of outer mitochondrial membrane 20 homolog (yeast) |
| NCBI Official Symbol: | TOMM20 |
| NCBI Official Synonym Symbols: | MAS20; MOM19; TOM20 |
| NCBI Protein Information: | mitochondrial import receptor subunit TOM20 homolog; mitochondrial 20 kDa outer membrane protein; outer mitochondrial membrane receptor Tom20; translocase of outer mitochondrial membrane 20 homolog type II |
| UniProt Protein Name: | Mitochondrial import receptor subunit TOM20 homolog |
| UniProt Synonym Protein Names: | Mitochondrial 20 kDa outer membrane protein; Outer mitochondrial membrane receptor Tom20 |
| Protein Family: | TOMM20-like protein |
| UniProt Gene Name: | TOMM20 |
| UniProt Entry Name: | TOM20_HUMAN |
 | Western blot analysis of extracts of various cell lines, using TOM20 antibody (CAB6774) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug TOM20 antibody (CAB6774). Western blot was performed from the immunoprecipitate using TOM20 antibody (CAB6774) at a dilition of 1:1000. |
 | Immunohistochemistry of paraffin-embedded human colon using TOM20 antibody (CAB6774) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat ovary using TOM20 antibody (CAB6774) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse heart using TOM20 antibody (CAB6774) at dilution of 1:100 (40x lens). |
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