Anti-TOM1 Antibody (CAB9273)
- SKU:
- CAB9273
- Product type:
- Antibody
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-TOM1 Antibody |
| Antibody SKU: | CAB9273 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 314-493 of human TOM1 (NP_001129204.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse kidney, Mouse liver, Rat kidney, Rat liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 314-493 of human TOM1 (NP_001129204.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | SEAE PAAD LIDM GPDP AATG NLSS QLAG MNLG SSSV RAGL QSLE ASGR LEDE FDMF ALTR GSSL ADQR KEVK YEAP QATD GLAG ALDA RQQS TGAI PVTQ ACLM EDIE QWLS TDVG NDAE EPKG VTSE GKFD KFLE ERAK AADR LPNL SSPS AEGP PGPP SGPA PRKK TQEK DDDM LFAL |
| Gene ID: | 10043 |
| Uniprot: | O60784 |
| Cellular Location: | Cytoplasm, Membrane, Peripheral membrane protein |
| Calculated MW: | 48kDa/50kDa/53kDa |
| Observed MW: | 54kDa |
| Synonyms: | TOM1 |
| Background: | This gene was identified as a target of the v-myb oncogene. The encoded protein shares its N-terminal domain in common with proteins associated with vesicular trafficking at the endosome. It is recruited to the endosomes by its interaction with endofin. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| NCBI Summary: | This gene was identified as a target of the v-myb oncogene. The encoded protein shares its N-terminal domain in common with proteins associated with vesicular trafficking at the endosome. It is recruited to the endosomes by its interaction with endofin. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008] |
| UniProt Code: | O60784 |
| NCBI GenInfo Identifier: | 1519241534 |
| NCBI Gene ID: | 10043 |
| NCBI Accession: | NM_005488.3 |
| UniProt Related Accession: | O60784 |
| Molecular Weight: | |
| NCBI Full Name: | Homo sapiens target of myb1 membrane trafficking protein (TOM1), transcript variant 1, mRNA |
| NCBI Synonym Full Names: | target of myb1 membrane trafficking protein |
| NCBI Official Symbol: | TOM1 |
| NCBI Protein Information: | target of Myb protein 1 |
| UniProt Protein Name: | Target of Myb protein 1 |
| Protein Family: | TOM1-like protein |
| UniProt Gene Name: | TOM1 |
| UniProt Entry Name: | TOM1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using TOM1 antibody (CAB9273) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of 293T cells using TOM1 antibody (CAB9273) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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