Anti-TNNT1 Antibody (CAB10354)
- SKU:
- CAB10354
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-TNNT1 Antibody |
| Antibody SKU: | CAB10354 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-262 of human TNNT1 (NP_001119604.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HT-1080, A-549, SW480 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-262 of human TNNT1 (NP_001119604.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MSDT EEQE YEEE QPEE EAAE EEEE APEE PEPV AEPE EERP KPSR PVVP PLIP PKIP EGER VDFD DIHR KRME KDLL ELQT LIDV HFEQ RKKE EEEL VALK ERIE RRRS ERAE QQRF RTEK ERER QAKL AEEK MRKE EEEA KKRA EDDA KKKK VLSN MGAH FGGY LVKA EQKR GKRQ TGRE MKVR ILSE RKKP LDID YMGE EQLR EKAQ ELSD WIHQ LESE KFDL MAKL KQQK YEIN VLYN RISH AQKF RKGA GKGR VGGR WK |
| Gene ID: | 7138 |
| Uniprot: | P13805 |
| Cellular Location: | |
| Calculated MW: | 30kDa/31kDa/32kDa |
| Observed MW: | 33kDa |
| Synonyms: | TNNT1, ANM, NEM5, STNT, TNT, TNTS, troponin T1 |
| Background: | This gene encodes a protein that is a subunit of troponin, which is a regulatory complex located on the thin filament of the sarcomere. This complex regulates striated muscle contraction in response to fluctuations in intracellular calcium concentration. This complex is composed of three subunits: troponin C, which binds calcium, troponin T, which binds tropomyosin, and troponin I, which is an inhibitory subunit. This protein is the slow skeletal troponin T subunit. Mutations in this gene cause nemaline myopathy type 5, also known as Amish nemaline myopathy, a neuromuscular disorder characterized by muscle weakness and rod-shaped, or nemaline, inclusions in skeletal muscle fibers which affects infants, resulting in death due to respiratory insufficiency, usually in the second year. Multiple transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | TNNT1: Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Defects in TNNT1 are the cause of nemaline myopathy type 5 (NEM5); also known as Amish nemaline myopathy (ANM). A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. Nemaline myopathy type 5 is a severe and progressive form common among Old Order Amish. Affected infants display tremors with hypotonia and mild contractures of the shoulders and hips. Proximal contractures progressively weaken and a pectus carinatum deformity develops before children die of respiratory insufficiency, usually in the second year. Belongs to the troponin T family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Contractile Chromosomal Location of Human Ortholog: 19q13.4 Cellular Component: cytosol; troponin complex Molecular Function:protein binding; tropomyosin binding; troponin T binding Biological Process: muscle filament sliding; negative regulation of muscle contraction; skeletal muscle contraction; slow-twitch skeletal muscle fiber contraction; transition between fast and slow fiber Disease: Nemaline Myopathy 5 |
| NCBI Summary: | This gene encodes a protein that is a subunit of troponin, which is a regulatory complex located on the thin filament of the sarcomere. This complex regulates striated muscle contraction in response to fluctuations in intracellular calcium concentration. This complex is composed of three subunits: troponin C, which binds calcium, troponin T, which binds tropomyosin, and troponin I, which is an inhibitory subunit. This protein is the slow skeletal troponin T subunit. Mutations in this gene cause nemaline myopathy type 5, also known as Amish nemaline myopathy, a neuromuscular disorder characterized by muscle weakness and rod-shaped, or nemaline, inclusions in skeletal muscle fibers which affects infants, resulting in death due to respiratory insufficiency, usually in the second year. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P13805 |
| NCBI GenInfo Identifier: | 1174800 |
| NCBI Gene ID: | 7138 |
| NCBI Accession: | P13805.4 |
| UniProt Secondary Accession: | P13805,O95472, Q16061, Q5U0E1, |
| UniProt Related Accession: | P13805 |
| Molecular Weight: | 31,242 Da |
| NCBI Full Name: | Troponin T, slow skeletal muscle |
| NCBI Synonym Full Names: | troponin T1, slow skeletal type |
| NCBI Official Symbol: | TNNT1 |
| NCBI Official Synonym Symbols: | ANM; TNT; NEM5; STNT; TNTS |
| NCBI Protein Information: | troponin T, slow skeletal muscle |
| UniProt Protein Name: | Troponin T, slow skeletal muscle |
| UniProt Synonym Protein Names: | Slow skeletal muscle troponin T; sTnT |
| Protein Family: | Troponin |
| UniProt Gene Name: | TNNT1 |
| UniProt Entry Name: | TNNT1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using TNNT1 antibody (CAB10354) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s. |
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